Ultrasound-Mediated BDNF Gene Transfection To The Rat Retina And Visual Cortex Enhanced The RGC Protection Effect After Optic Nerve Injury

ObjectiveTransfected BDNF gene to the rat retina and visual cortex by ultrasound-mediated and estimate the protection effect on the RGC.MethodsSD male rats 88, were randomly divided into six groups, namely A normal groups (8), B sham operation groups (16), C control groups (16), D eyes transfection groups ( 16), E brain transfection groups (16), F co-transfection groups (16). After 1 week and 2 weeks of the injury, use retrograde fluorogold labeled RGC to counte the cell number, immunohistochemistry to test caspase-3, PERG to text the visual function of RGC.Results1 week and 2 weeks after the nerve injury, F Groups RGC number was 409.1±42.10 and 390.8±35.34(A Groups's 86.0% and 82.1%),significantly higher than other groups. Caspase-3 text showed that F groups at different time points, apoptotic cell number were less than other experimental groups. PERG responses for all the treated groups were normal at 1 week after injury; however, at 2 weeks, only F groups remained so(7.33±0.58μV).ConclusionUltrasound-mediated BDNF gene transfection to the rat retina and visual cortex could significantly enhance the RGC protection effect after optic Nerve Injury...