The Effect Of Chronic Intermittent Hypoxia On Expression Of HIF-α, TNF-α, IL-6 In HepG2 Cell

ObjectiveObstructive sleep apnea hypopnea syndrome (OSAHS) is a potentially dangerous disease, the incidence rate of 2%to 4%higher incidence in the elderly. OSAHS is closed to coronary heart disease, hypertension and atheroscleros. Chronic intermittent hypoxia is one of the main pathogenic mechanism. Chronic intermittent hypoxemia is possibly responsible for triggering oxidative stress and inflammatory reaction in OSAHS. It has been found that chronic intermittent hypoxia can lead to abnormal lipid metabolism, lipid metabolic disorders and cardiovascular disease. The research is to human liver cancer cells(HepG2 cells) by chronic intermittent hypoxia and chronic continuous hypoxia intervention, to discuss the relations between HIF-lα, IL-6, TNF-αand chronic intermittent hypoxia and the affection of chronic intermittent hypoxia on inflammatory reaction. The purpose is to provide essential laboratory evidences and theoretical basis for the clinical treatment.Methods1.HepG2 cells were used to set up the cell model.The experiment was consisted of 5 groups according to different degrees and models:21% oxygen concentration;continuous hypoxia of 10%and 5%; intermittent hypoxia of 5%and 10%.2.Laboratory testing:Investigate the level of HIF-1α, IL-6, TNF-αconcentration by enzyme-linked immunosorbent assay of serum.Resultsl.The level of HIF-1α:5%intermittent hypoxia group compared to 10%intermittent hypoxia group showed no significant difference (p> 0.05); 5%continuous hypoxia group compared to 10%continuous group showed no significant difference (p>0.05);5%intermittent hypoxia and 5%continuous hypoxia compared to HIF-la were significantly increased (p<0.05); 10%intermittent hypoxia and 10%continuous hypoxia compared to HIF-la was significantly increased (p< 0.05); 21% oxygen group and other groups compared to HIF-la was significantly decreased (p<0.05);2.The level of IL-6:5%intermittent hypoxia and 5%continuous hypoxia compared to IL-6 was significantly increased (p<0.05); 10% intermittent hypoxia and 10%continuous hypoxia compared to IL-6 were significantly increased (p<0.05); 5%intermittent hypoxia and 10% intermittent hypoxia compared to IL-6 were significantly increased (p< 0.05); 5%continuous hypoxia and 10%continuous hypoxia compared to IL-6 was significantly increased (p<0.05); 21%oxygen group and other groups compared to IL-6 were significantly decreased (p<0.05);3. The level of TNF-a:5%intermittent hypoxia and 5%continuous hypoxia compared to TNF-a was significantly increased (p< 0.05); 10% intermittent hypoxia and 10%continuous hypoxia compared to TNF-a was significantly increased (p<0.05);5%intermittent hypoxia and 10% intermittent hypoxia compared to TNF-a was significantly increased (p< 0.05); 5%continuous hypoxia and 10%continuous hypoxia compared to IL-6 was significantly increased (p<0.05);21%oxygen group and other groups compared to TNF-a were significantly decreased (p<0.05);4. This study HIF-1α, TNF-α, IL-6, comparisons between the positive correlation:HIF-la and IL-6 compared positively correlated (r=0.287, p< 0.05); HIF-1αand TNF-a Comparison of positive correlation (r=0.273, p<0.05); IL-6 and TNF-a compared positively correlated (r=0.713, p<0.05)Conclusion1.Chronic intermittent hypoxia and chronic continuous hypoxia can lead to the activation of HIF-1αand the increasing content of IL-6,TNF-a in HepG2 cell.2.The effect of chronic intermittent hypoxia on expression of TNF-a,IL-6 in HepG2 cell is more obvious than the effect of Chronic continuous hypoxia,and the effect of Chronic continuous hypoxia on expression of HIF-1αin HepG2 cell is more obvious than the effect of chronic intermittent hypoxia.