Effect Of Willed Movement Therapy On The Expression Of NT-3 And GAP-43 In Penumbra Area Of Rats With Cerebral Ischemia Reperfusion

Backgroud and Objective Willed movement can increase synaptic plasticity and improve never functional recovery. This study will establish the model of middle cerebral artery occlusion in SD rats and intervene in willed movement therapy, then observe the effect of willed movement therapy on the expression of neurotrophin 3 (NT-3) and growth associated protein 43 (GAP-43) in rats with cerebral ischemia-reperfusion and to investigate the neuroprotection mechanism of willed movement therapy in nerve regeneration and repair.Methods Fifty-four healthy male Sprague-Dawley (SD) rats weighting 240g-260g were selected and then randomly divided into three groups:a middle cerebral artery occlusion group(MCAO group), an environment modification group (EM group) and a willed movement therapy group (WM group). Each group has 18 rats. The model of middle cerebral artery occlusion in SD rats was established by nylon monofilament suture. We evaluated the neurological defects and decapitated the rats on day 3,7 and 15 after the reperfusion. Meanwhile, the expressions of NT-3 and GAP-43 in the cerebral ischemia penumbra were measured by immunohistochemistry (IH)Results1. Scores of neurological deficits:There were no significant differences among three groups at 3 and 7days after the reperfusion(P> 0.05). Compared with the MCAO group and the EM group, the grade of neurological defects in the rats was lower (P<0.05) in the WM group15 days after the reperfusion.2. There were no significant differences in the expression of NT-3 among three groups at 3 days after the reperfusion(P>0.05). Compared with the MCAO group and the EM group, the expressions of NT-3 were higher (P<0.05) in the WM group at 7 and 15 days after the reperfusion. But there was no significant difference in the expression of NT-3 between the MCAO group and the EM group(P>0.05).3. There were no significant differences in the expression of GAP-43 among three groups at 3 days after the reperfusion(P>0.05). Compared with the MCAO group and the EM group, the expressions of GAP-43 were higher (P<0.05) in the WM group at 7 and 15 days after the reperfusion. But there was no significant difference in the expression of GAP-43 between the MCAO group and the EM group(P>0.05).4. The expression of NT-3 was positively correlated with that of GAP-43.ConclusionsWilled movement therapy increases the expression of NT-3 and GAP-43 in the cerebral ischemia penumbra in rats with cerebral ischemia-reperfusion, which is probably related to nerve regeneration and repair.