| Objective To investigate the effect of 17β-Estradiol on the intestinal motility of ovariectomized Sprague Dawley (SD) rats and the possible mechanism.Methods After established animal model of ovariectomized SD rats, the difference of small intestinal propulsive rates in variant groups which were dealt with different concentrations of 17β-estradiol in order to find out the change of small intestinal transmission in vivo; Changes of contraction tension of small intestine smooth muscles pieces, which were obtained from the ovariectomized SD rats, were recorded by the BL-420E biological function experiment system in vitro after muscles pieces were treated by different concentrations of carbachol,17(3-estradiol and estrogen receptor antagonist Fulvestrant.Results1.The small intestinal propulsive rate in small intestine of ovariectomized SD rats was decreased after 17β-estradiol treatment, the higher concentration of 17β-estradiol, the stronger inhibition was.2.10-4mol/l of carbachol could increase contraction tension of the small intestine smooth muscles while sequent 17β-estradiol could inhibit the elevated contraction, furthmore the inhibition of 17β-estradiol was increased with the concentration enhancement. But the above inhibition of 17β-estradiol could be significantly eliminated by the estrogen receptor antagonist Fulvestrant.Conclusions1. In vivo 17β-estradiol could inhibit small intestine transmission of ovariectomized SD rats, and the effect was correlated with the concentration of 17p-estradiol.2. In vitro 17β-estradiol could inhibit the contraction tension enhancement of small intestine smooth muscles pieces from ovariectomized SD rats caused by carbachol, moreover the effect was correlated with the concentration of 17p-estradiol. This inhibition could be eliminated by Fulvestrant.3. The inhibition of 17β-estradiol may work through estrogen receptors on the small intestine smooth muscle in female SD rats.
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