Association Study Of Sheat Shock Protein 70-2 Gene Single Nucleotide Polymorphism And Diabetic Nephropathy

BackgroundHSPs (heat-shock proteins) are molecular chaperones synthesized under stress conditions, and are involved in renal cell survival and matrix remodelling in acute and chronic renal diseases.ObjectiveInvestigated whether the HSP70-2 gene polymorphisms affect susceptibility to DN (diabetic nephropathy) in patients with T2DM(Type 2 diabetes mellitus). To ensure the susceptibility gene for diabetic nephropathy, provide the possibility to treat patients with diabetic complications earlier, or even delay the onset of the complications.MethodsThe study group consisted of 80 patients with Diabetic nephropathy (DN). Two control subgroups involved 80 healthy individuals (HC) and 80 patients with T2DM who were free of nephropathy (DM). Subjects were genotyped for the HSP70-2+1538 A/G polymorphisms by PCR, followed by digestion with restriction endonucleases. Other laboratorial results such as blood glucose, HbAlc, lipid profiles were measured.ResultsThe genotype frequencies in the DN, DM and HC group were 84%,80.3%,76.8% for AG genotype, while they were 16%,19.7%,23.2% for GG genotype. There was no statistically difference between DN and DM group (χ2=0.329, p=0.566), and it was the same between DN group and HC group (χ2=1.081, p=0.346). The A allel frequency in the DN, DM and HC group were 42%,40.2%,38.4%, while the G allel frequency was 58%,59.8%,61.6% for the three groups. There was no statistically difference between DN and DM group (χ2=0.099, p=0.753), and it was the same between DN group and HC group (χ2=0.346, p=0.556). Multiple logistic analysis also confirmed that the HSP70-2 1538A/G polymorphism is not the risk factor of Diabetic Nephropathy.ConclutionsThe 1538A/G polymorphism of HSP70-2 gene is not significantly associated with type 2 diabetes with nephropathy in Hangzhou Chinese population. Both GG genotype and G allel are not the risk factor of the type 2 diabetic nephropathy.