Clinicopathological Pattern And Annexin A2 And Cdc42 Status In Patients Presenting With Differentiation And Lymphnode Metastasis Of Esophageal Squamous Cell Carcinomas

Objective:Annexin A2 and Cdc42 were identified by 2-dimentional electrophosis (2-DE) and MALDI-TOF-MS between esophageal squamous cell carcinomas (ESCC) and corresponding normal esophagus mucosa in our previous study. To assess clinico-pathological pattern and Annexin A2 and Cdc42 status with respect to differentiation and lymphnode metastasis of ESCC. Methods:All ESCC tissues (as experimental group) and matched normal esophageal tissues (as control group) were obtained from surgical specimens of 175 patients with ESCC from 2000 to 2008 in the Affiliated Hospital, Xinjiang medical University, China. The expression of Annexin A2 and Cdc42 in 22 pairs of fresh ESCC and matched tissues were detected by qRT-PCR and western-blot, respectively. And it was further confirmed by immunohistochemistry with 175 pairs of formalin-fixed, parafin-embeded ESCC. Results:The expression of Annexin A2 was significantly lower in the ESCC (0.02±0.02) than that in corresponding control (0.06±0.06) on mRNA level (T test,p<0.05). On protein level, the expression of Annexin A2 was significantly lower in the ESCC (0.81±0.36) than that matched tissues (0.95±0.42) (T test,P<0.05). By immunohistochemistrical analysis,82.3%(144/175) of the ESCC samples were showed lower expression of Annexin A2 protein compared with corresponding tissues 92.0%(161/175)(χ2test, p<0.05). The expression of Cdc42 was significantly higher in the ESCC (0.21±0.14) than that in corresponding control (0.16±0.12) on mRNA level (T test, p<0.05). On protein level, the expression of Cdc42 was significantly higher in the ESCC (0.83±0.35) than that matched tissues (0.75±0.24), by immunohistochemistrical analysis,73.7%(129/175) of the ESCC samples were showed higher expression of Cdc42 protein compared with corresponding tissues 62.9% (110/175)(x2 test, p<0.05). Additionally, Annexin A2 and Cdc42 expression was significantly correlated with lymphoid node metastasis (p< 0.05) and pathological differentiation (p<0.05). Whereas it was no significant correlation (p>0.05) could be appreciated between them and ESCC morphology. Conclusion:The aberrant expression of Annexin A2 and Cdc42 played a role in carcinogenesis, differentiation and metastasis of ESCC, implying its potential target for clinical biomakers in differentiation and lymph node metastasis.