Establishment Of Hepatocarcinoma Carrying Abnormal Savda Animal Model And It's Morphological Study

Objective To establish combining hepatocarcinoma disease and abnormal savda syndromes rats model, and investigate the effect of abnormal savda on inducing and developing process of hepatocirrhosis phase in this model. Methods Under the direction of uighur traditional medicine, multi complcated induction methods were used to establish abnormal savda syndromes rats model for three weeks, using DEN furtherly to establish combining hepatocarcinoma and abnormal savda disease rats model till hepatocirrhosis occurs, the animals were anesthetized at 5,7,9,11 weeks under the eyes of venous blood, the appearance of rat liver In HE staining under light microscope and transmission electron microscopy, liver morphology and cell ultrastructure were observed dynamically. Results both in the model control group and combining disease and abnormal savda syndromes model group there are the pathological changing process of hepatoxic lesion, hepatic proliferation, hepatocirrhosis, but the pathological changes of the liver surface in the combining disease and abnormal savda syndromes model group is faster than model control group. At the same time point, the dropsical level of liver cell, typeless hyperplasia, fake foliole level in liver, the ultrastructural changes of decreasing hepatin and chondriosome in liver cells in combining disease and abnormal savda syndromes model group are than those in the model control group. Conclusion These findings indicate that in the process in which establishing abnormal savda disease rat model serving as a base, using DEN furthurly to establish combining hepatocarcinoma disease and abnormal savda syndromes rats model, the abnormal savda hilit accelerates the inducing and developing process of hepatocyte to hepatic Cellular proliferation to hepatocellular carcinoma and this process is similar to the process of humans hepatocarcinoma. It is also stable and reliable.