Preparation And Biological Performance Of β-tricalcium Phosphate Composite/Poly(D, L-lactic Acid)

Two composite were researched to repair nerve defect, and compared degradation performance and biocompatibility of two composite. Firstly, two different morphologies nanoscaleβ-tricalcium phosphate(P-TCP) were synthesized. then composite was prepared with the copolymer was physical mixed with PDLLA and two different morphologiesβ-TCP powder. Moreover, degradation performance of composite was researched by degradation experiment in vitro, and the biocompatibility of which was evaluated by cell experiments in vitro. And which was expected to have good cytocompatibility.Climate nanoscaleβ-TCP was synthesized by precipitation methods. Crystal phase, morphology, phase composition were charactered by X-ray diffraction(XRD), transmission electron microscopy(TEM), fourier transform infrared spectroscopy(FT-IR). It was found that it was high purity, crystalline grain tiny, fine crystalline and steady dispersivity. Lamellarβ-TCP was synthesized by dry process, which were charactered by X-ray diffraction, transmission electron microscopy, Fourier Transform Infrared Spectroscopy. It was found that it have fine crystalline, Reaction completely,the main crystalline was crystalline phase ofβ-TCP.According to study the preparation process of the composite and factors influence membrane performance, the composite membrane was prepared using ethyl acetate as solvent under 24℃and 60% humidity by solvent volatilization method. The range of molecular weight of PDLLA was selected as 1.5×105. The grain size of clavateβ-TCP power was 10～20nm. The structure and properties of the composite membrane were characterized. At the same condition, the composite membrane was prepared using methylene chloride by solvent volatilization method. The grain size of lamellarβ-TCP power was 50nm. And the structure and performance were charactered.The pH value of degradation medium, weight loss ratio of the composite membrane and the variation of Compressive strength were determinated during the degradation in vitro. The results showed that:β-TCP can partily neutralize the acidity which produced by degradation of PDLLA. Effectively regulate the pH value of degradation medium to keep at neuter. Soβ-TCP/PDLLA composite has better biodegradation properties than PDLLA. Compared with clavate composite, Lamellar composite was faster in pH value of degradation medium. Compared with lamellar composite, clavate composite materials was smaller in weight loss ratio and higher in compressive strength.After cultivating Glial cells on the surface of the composite, the cell activity and the surface feamre of the Glial cells were detected by the MTT experiments and scanning electron microscope. The experiments in vitro of Glial cells and materials showed that two kinds ofβ-TCP/PDLLA composite materials has better cell affinity than PDLLA, which can promote the Glial cells adhesion, proliferation and growth on the surface of composite.