Study On The Mechanisms Of Bushen Jiedu Huoxue Recipe For The Myelosuppression After Chemotherapy

Objeetive:To study the effects and the mechanisms of Bushen Jiedu Huoxue Recipe for the Myelosuppression after Chemotherapy.Methods:140 Clean grade Kunming mices. half-female and half-male,Divided into two parts:preventive experiment and therapeutic experiment.Preventive experiment:Totally 60 healthy Kunming mice were used, half-female and half-male, randomly divided into 3 groups:normal control group, model group, Chinese herb Preventive group with 20 mice in each group.The Chinese herb Preventive group was administered Bushen Jiedu Huoxue Recipe,Mice of normal control group and model group were given saline of the same volume by gastric perfusion.once a day for 10 days successively.Except the normal control group,mice in other groups received 100mg/kg cyclophosphamide (CTX) by intraperitioneal injection for 3 days successively from the 8th day. randomly abstract 10 mices for blood tests on the day before the establishment of model,and on the 1,3,5,7,10days after the establishment of model.The remaining 10 ones were killed to study the effects of the index of thymus gland and spleen; CFU-GM,CFU-E and BFU-E; the apoptosis of bone marrow cells;serum concentration of EPO and GM-CSF.Therapeutic Experiment:80 healthy Kunming mice were used and randomly divided into 4 groups:normal control group, model group, Chinese herb therapeutic group and positive control group with 20 mice in each group. The establishment of model was the same to the preventive experiment.Normal control group, model group, Chinese herb Preventive group were given accordingly medicine by gastric perfusion.once a day for 10 days successively after the establishment of model.The positive control group was administered rhG-CSF once daily by intraperitoneal injection for 3 consecutive days after the establishment of model. randomly abstract 10 mices for blood tests on the day before the establishment of model,and on the 1,3,5,7,10,14days after the 10 days successively intragastric administration. The remaining 10 ones were killed to study the effects of the index of thymus gland and spleen; CFU-GM,CFU-E and BFU-E;serum concentration of EPO and GM-CSF on the 11th day. The Chinese herb Preventive groupResults:1. the establishment of model The WBC and PLT in model group of both the preventive and therapeutic experiment were obviously decreased, which meet the performance of myelosuppression after chemotherapy.2.the effect of the preventive and therapeutic experimentIn the preventive experiment, Of the model group,the WBC dropped to the lowest on the first day after modeling.come to normal on the 5th day. The PLT dropped to the lowest on the 5th day after modeling.come to normal on the 10th day. The degree of reduction of the Chinese herbpreventive group was lower than the model group. (P<0.01 or P<0.05)In the therapeutic experiment,The blood cell of the model group was similar to the preventive experiment.The WBC of Chinese herb therapeutic group and positive control group was higher than the model group;However,the The degree of reduction of PLT of Chinese herb therapeutic group was lower than the model group and the positive group.and it come to normal at the 7th day,which is higher than the model group and the positive group.3. Analysis of mechanism3.1 the index of thymus gland of the preventive group was higher than the model group.in the therapeutic experiment, the index of thymus gland of the therapeutic group was lower than the normal group,but higher than the model group On the contrary was the index of the spleen.3.2 serum concentration of GM-CSF of the preventive group was still higer than the model group,although lower than the normal group.The EPO of each group have on changes.in the therapeutic experiment,the EPO and GM-CSF of the therapeutic group were significantly higer than that of the model group.However,the superiority of the positive group was only in GM-CSF.3.3BMNC and CFU-GM of the preventive group was lower than the normal group.,but still higer than the model group. TheCFU-E and BFU-E have on changes. in the therapeutic experiment,the BMNC and HPC of the therapeutic group were significantly higer than that of the model group.However,the superiority of the positive group was only in BMNC and CFU-GM. 3.4the apoptosis of bone marrow cells. there was no significant different between the preventive group and the normal group.which was lower than that of the model group (P<0.01). duing to the apoptosis happened among 24 hours,the index wasn't be done in the therapeutic experiment.Conclusion:1. the myelosuppression mice model can be successfully established by intraperitioneal inject CTX.2. Both the preventive administration and the therapeutic administration are helpful to the recovery and the reduction of the myelosuppression3. the Bushen Jiedu Huoxue Recipe can raise the index of thymus gland and depress that of spleen.4. the preventive administration elevate the serum concentration of GM-CSF,BMNC and GM-CSF. the therapeutic administration elevate the serum concentration of GM-CSF, BMC and CFU-GM. CFU-E and BFU-E.5. the preventive administration can depress the apoptosis of the bone marrow cells.