Effect Of San WuBaiSan On The Apoptosis And Survivin Expression Of Gastric Cancer Cell Lines SGC-7901

Objective:To investigat the effect of SanWuBaiSan-containing serum on the apoptosis and survivin expression of human gastric cancer cell lines SGC-7901.Methods:To choose the human gastric cancer cell lines SGC-7901, and SGC-7901 cells were treated by different concentrations of SanWuBaiSan-containing serum.The apoptosis was measured by fluorescence staining after the SGC-7901 cells were treated by SanWuBaiSan-containing serum,survivin expression of the SGC-7901 cells treated by SanWuBaiSan-containing serum were detected by RT-PCR.Results:1. After the SGC-7901 cells treated by different concentrations of SanWuBaiSan-containing serum.compares with the physiological saline control group, there was a increasing tendency on the apoptosis of SGC-7901 cells,and as the concentration increasing and time elongation,the apoptosis of SGC-7901 cells was increased gradually. 2.There was a decreasing tendency in different degrees on the survivin expression after the SGC-7901 cells treated by different concentrations of SanWuBaiSan-containing serum, Compares with the physiological saline control group, there was a statistics significance difference (P<0.05),and as the concentration increasing and time prolongation, the survivin expression of SGC-7901 cells was decreased gradually.Conclusions:1. SanWuBaiSan can induce apoptosis of SGC-7901 cells in a definite period of dose and time, and it depends on the definite dose and time.2.Different concentrations of SanWuBaiSan-containing serum can inhibit survivin expression of gastric cancer SGC-7901 cells, and expression with dose-dependence and time-dependence. The results of this study indicate that the SanWuBaiSan-containing serum could induce apoptosis of SGC-7901 cells and its mechanism may be related to inhibiting the expression of survivin.