Abnormal Expression Of PTPRT In The Temporal Lobe Epileptic Foci: A Study On The Pathogenesis Of Intractable Epilepsy

Objectives:PTPRT is mainly expressed in the central nervous system. PTPRT can regulates synapse formation by interacting with cell adhesion molecules. Overexpressed of PTPRT increased the density of dendritic spines and excitatory and inhibitory synapses. Conversely, knockdown of PTPRT resulted in decrease in the number of dendritic spines and excitatory synaptic transmission. The function of PTPRT indicates it may play a role in the epilepsy. In order to verify our speculation, we investigate the expression changes of the PTPRT in temporal lobe epileptic foci from the experiment animal and epileptic patients and to approach the relationship between PTPRT and epileptogenesis.Methods:We acquired randomly 30 temporal lobe tissue from the brain tissue of intractable epilepsy patients collected by the first affiliated hospital of Chongqing medical college, then acquired 10 temporal lobe tissue from trauma surgery patients which composed the control group. Sixty-three healthy 6-8 years SD rats provided by the experimental animal center of Chongqing university were divided to seven groups, six groups were injected lithium -pilocarpine and obtained epilepsy, the other one was control group. The six experiment groups respectively contained 9 rats at 6h,1d,7d,14d,30d,60d days post-seizure. We get the epilepsy lobe tissue from the experiment and control groups as research objects, using the immunohistochemistry, immunofluorescence and Western blot analysis to assess the expression of PTPRT and its changes.Results:In the temporal lobe tissue of intractable patients and control groups, PTPRT was mainly expressed in the neurons. PTPRT significantly increased in intractable epilepsy patients than the control group. PTPRT was mainly expressed in the neurons in the temporal lobe brain tissue of the rats in the control group and experiment groups. Compared with control groups, PTPRT expression in the temporal lobe tissue was rising within 24h post-seizure, and decreased 1 and 2 weeks post-seizure, then it is on the rise 1 and 2 months post-seizure.Conlusions:Through researches on the PTPRT expression in the temporal lobe brain tissue in the intractable epilepsy patients and animal models, together with the function of PTPRT, we presume that the PTPRT play a role in the synapses reorganization and moss fiber sprouting, participating in the reconstruction of the neural network which led to the intractable epilepsy.