Patients With COPD Peripheral Skeletal Muscle Atrophy Related Structure Protein Preliminarily Analyzed

Objective:Using two-dimensional gel electrophoresis of fingerprint spectra-peptide quality of proteomics method to establish the healthy elderly, chronic obstructive pulmonary disease and the muscular atrophy shrinking two-dimensional gel electrophoresis mapping, identification, appraisal and COPD peripheral skeletal muscle atrophy, this paper discusses the structure protein related to the pathogenesis of muscular atrophy of COPD.Methods:Twenty-seven cases Will list is divided into health group (9 cases), COPD skeletal muscle atrophy group (9 cases) COPD skeletal muscles not shrinking group (9 cases), first lung function were, body composition, blood gas analysis, quadriceps muscle contraction forces determine maximum active. Then by two-dimensional gel electrophoresis COPD peripheral skeletal muscle atrophy, the shrinking of the healthy elderly patients and normal outside the quadriceps muscle tissue of total protein, using scanning images get 2-DE PDQuest image maps that will compare electrophoresis software of abnormal protein points, according to the protein point hike in two times more than 2 times the drop or points to first click on standards, combined with 2-DE atlas, through the atlas of the depth and size glue point selection have differences identified of protein expression point; Application of laser desorption matrix auxiliary/ionization time-of-flight mass spectrometry meter obtained corresponding peptide quality fingerprint spectra, finally, using the control software combines NCBInr database retrieval appraisal related proteins.Results:1. Patients with COPD skeletal muscle atrophy j clinical features: COPD skeletal muscle atrophy group a COPD skeletal muscle atrophy group the course of a long, not in the COPD skeletal muscle wasting group weight, body mass index, to fat index, quadriceps muscle weeks diameter, quadriceps muscle contraction forces maximum active control are healthier than atrophy group, COPD skeletal muscle low (P<0.01), COPD skeletal muscle atrophy group and healthy controls than in weight, to fat index was statistically significant (P=0.36), COPD skeletal muscle atrophy in mass of the index, the quadriceps muscle weeks diameter, maximum active quadriceps muscle contraction forces more healthy controls low (P<0.05). COPD skeletal muscle atrophy group in the lung function measurements FEV1/pred%, FEV1/FVC% healthier than control group, COPD skeletal muscle atrophy group (P<0.05) low, COPD skeletal muscle atrophy group is not healthy group low (P<0.05). Three groups in blood gas analysis results PaO2 statistically significant (P<0.01), but no difference PaCO2 aspects of skeletal muscle atrophy, COPD PaO2 relatively healthy controls of skeletal muscle atrophy group, COPD not low (P<0.01).2.Get resolution and repeatability are good two-dimensional gel electrophoresis, and identified may occur with COPD muscular atrophy eight kinds of related closely related structure protein:actin, alpha skeletal muscle, actin, alpha, cardiac muscle, isoform CRA_b,myosin light chain 6B,myoglobin isoform,actin, alpha, cardiac muscle, isoform CRA_c,Myosin regulatory light chain-2,ventricular/cardiac muscle isoform,myosinlightchain1/3,skeletal muscleisoform 3f,myosin, heavy polypeptide 7, cardiac muscle, beta, isoform CR.3. In health and atrophy group, compared with healthy group, myosin, heavy polypeptide 7, cardiac muscle, beta, isoform CR in COPD happen for raised; atrophic performance Health and the shrinking group compared with healthy, myoglobin isoform expression for raised; With the shrinking groups in atrophy, relative to atrophy group is concerned, actin, alpha, cardiac muscle, isoform CRA_c in the shrinking performance for to rise, actin, alpha skeletal muscle performance for the cut in the shrinking; Through to health and atrophy, non atrophy three groups after we found together to compare:group compared with healthy, myosin light chain 6B, Myosin regulatory light chain 2, ventricular/cardiac muscle isoform, myosinlightchain1/3,skeletal muscle isoform 3f, myoglobin isoform with the atrophy in atrophy group were raised, only displays for actin, alpha, cardiac muscle, isoform CRA_b performance, and in not to cut group, the protein atrophy raised or cut the extent of change is more apparent, myosinlightchain1/3,skeletal muscle,isoform 3f and myosin light chain 6B group in atrophic changes is obvious.Conclusion:COPD accompanied obvious peripheral skeletal muscle function barrier, its essence including related structure protein quantity and quality change, change and the related structure protein peripheral skeletal disorders in COPD progression, related to different stages, the above structure protein expression has significant changes in the number may be patients with COPD in long-term anoxic, oxidatie stress, salt and water electrolyte acid-base disorders, malnutrition and systemic inflammation and skeletal muscle type conversion multi-factor under the combined action of results. Proteomics for study patients with COPD peripheral muscular atrophy provide theoretical foundation and the mechanism of research direction.