Formative And Adhesional Differences Of Calcium Oxalate Crystals Modulated By Different States Of (Nomal, Injured, Repaired) Vero Cells

Renal epithelial cell injury is considered as one of the important reasons of the formation of urolithiasis. Injured cells not only promoted the formation of calcium oxalate monohydrate (COM) crystals, but also greatly increased the adhesion of COM crystals, thus increasing the risk of stone formation. Therefore, the formation of urolithiasis could be inhibited if the injured renal epithelial cell was repaired effectively. Based on this, to provide a new insight in drugs exploitation of renal stone therapy and prevention of urolithiasis, Scanning electron microscopy, X-ray powder diffraction (XRD), Flow cytometry (FCM), CCK-8 kit and superoxide dismutase (SOD) kits were used to investigate the modulation of degraded soybean polysaccharide (SPS) to the growth of calcium oxalate (CaOxa) crystals in the urine and the repair of degraded SPS to African green monkey renal epithelial (Vero) cells.1. Low molecular weight SPS was obtained by the oxidative degradation of hydrogen peroxide (H2O2). The changes of average relative molecular weight, carboxyl content and sugar content of SPS before and after degradation were compared. The differences of modulation of SPS before and after degradation to the growth of CaOxa crystals were studied in the urine. The results showed that all of the undegraded and degraded SPS could inhibit the growth of calcium oxalate monohydrate (COM) crystals and induce the formation of calcium oxalate dihydrate (COD) crystals. However, compared with that of undegraded SPS, the degraded SPS showed much stronger inhibition on the growth of COM crystals. The COD crystals with smaller size were formed when the degraded SPS was at low concentration.2. After injured cells were repaired by degraded SPS (referred to as repaired cells), the cell growth state, the cell viability, the extracellular SOD activity, the intracellular mitochondrial membrane potential of normal cells, injured cells and repaired cells and the differences of CaOxa crystals compositions induced by these three typological cells in CaOxa supersaturated solution were investigated respectively. The results indicated that injured cells could be repaired by degraded SPS and the formation of COM crystals with the sharp edges could be reduced, while more blunt COD crystals could be induced by repaired cells. Further studies demonstrated that the soluble and aggregative ability to CaOxa crystals caused by normal cells, injured cells and repaired cells were different.3. The adhesion of normal cells, injured cells and repaired cells to COM and COD microcrystalline were also investigated. The results showed that the adhesive capacity of these three typological cells to CaOxa microcrystalline were as follow:injured cells> repaired cells> normal cells. Larger size CaOxa crystallite aggregates were caused by injured cells easily. But they could be decreased significantly by repaired cells, reducing the risk of stone formation.