The Research On The Cytotoxic Constituents From Two Strains Of Marine-derived Actinomyces

Objective To study the bioactive secondary metabolites from two strains of marine-derived Actinomycetes Micromonospora sp. (M2DG17) and Streptomyces sp. (0616121). Methods By bioassay-guided fractionation, various chromatographic and spectroscopic methods were applied for the isolation and structural identification of pure compounds from the broth of Micromonospora sp. (M2DG17) and Streptomyces sp. (0616121). Results Twelve compounds were obtained from the broth of Micromonospora sp. (M2DG17) and their structures were identified as 3-hydroxymethyl-β-carboline (compound 1),3-methyl-β-carboline (compound 2), P-carboline (compound 3),3-Hydroxy-2-methyl-4-pyrone (compound 4), Cyclo-(L-Pro-L-Phe) (compound 5), Cyclo-(L-Pro-L-Leu) (compound 6), Cyclo-(L-Pro-L-Val) (compound 7), and Cyclo-(L-Pro-L-Ile) (compound 8), Phthalate(2-ethyl)hexyl (compound 9), Isobutyl phthalate (compound 10), Phenylacetic Acid (compound 11), and Daidzein (compound 12), respectively. Compound 1-5,7-10 were isolated from the genus of Micromonospora sp. (M2DG17) for the first time, and compound 2 showed weak inhibitory effect on HCT 116 cell lines with IC50 values of 65.0μM. Nine compounds were obtained from the broth of Streptomyces sp. (0616121) respectively., and four compounds were identical with compounds 5-10. The other five ones were identified as Dibutyi phthalate(compound 13), Cyclo-(D-Pro-L-Val) (compound 14), Cyclo- (L-Pro-L-Met) (compound 15), Cyclo- (D-Pro-L-Ile) (compound 16), Cyclo (Ala-Leu) (compound 17). Compound 15 were isolated from the genus of Streptomyces sp. (0616121) for the first time.