The Clinical Application Of Fluorescence In Situ Hybridization In Detecting The Chromosomal Abnormality Of Spontaneous Miscarriage Tissue

【Objective】To explore an optimal method of sample pretreatment, operating sequence and quality control of chorionic villus cells or fetal tissue by fluorescence in situ hybridization(FISH).To discuss the incidence of chromosomal abnormalities of miscarriage tissue collected from pregnant women in the first, second and last trimester in cases of spontaneous abortion or fetal death.【Methods】Establish a standardized operating platform, detection the chromosomal abnormalities of 156 cases of complete miscarriage fetal tissue by FISH.[Results] Of the 156 cases, FISH was successfully tested in all cases(100%).There were 132 cases of the first trimester,18 cases of the second trimester and 6 cases of the last trimester. In which abnormal embryo karyotypes were identified in 82 cases (52.6%). Trisomy was the most frequent embryo karyotype abnormalities in these cases. The proportion of fetal chromosomal abnormalities in the advanced maternal age group was 63.2%(24/38),which in the non-advanced maternal age group was 49.2%(58/118). There was not statistically significant difference between the two groups. Chromosomal aberration occurred in 67 cases (53.6%) of the first spontaneous abortion and in 15 cases (48.4%) of recurrent abortions. Female fetuses accounted for 60.9%(95/156)in the spontaneously aborted fetuses, while male fetuses accounted for 39.1%(61/156). The incidence of chromosomal abnormalities in the two groups were 45.3% and 63.9%, there were significant differences between the two groups (P=0.023).【Conclusion】Embryo chromosomal abnormality is the most important reason of spontaneous abortion, and fluorescence in situ hybridization is a rapid, accurate and efficient way helps identify the causes of abortion and ensure the success of the next pregnancy. It has a good prospect of clinical application.