| The ligustilide is one classic representative of phthalide compound, which has some pharmacological effects on spasmolysis, smooth wheezing and clam. The study aims to obtain some Chinese medicine extract which contain copious ligustilide, to evaluate the anti-inflammatory and agalgesic effects of Ligustilide. And to study the therapeutic effects of ligustilide on rodent's experimental dysmenorrhea and to reveal the ligustilide's analgesic mechanism. Here are the research process and results:1. The preparation of ligustilideA best extraction way, which can obtain the most ligustilide, was chosen from the ultrasonic extraction, cold-maceration, thermal circumflence extraction, soxhlet extraction and Steam Distillation. Then, the crude extract that went through two purification steps turned into the purer one, which contained ligustilide at least 85%. This can be used in drug efficacy study.2. Anti-inflammatory and analgesic effects of ligustilideThe Kunming mice and SD rats are served as test animals. The pharmacodynamic effects were obsersed in 2 analgesic tests and 4 inflammatory experimental models, including:(1) Hot plate test. (2) Writhing reaction induced by acetic acid in mice. (3) Increased permeability of abdominal cavity induced by acetic acid in mice. (4) Auricular edema induced by dimethy benzene. (5) Paw swelling caused by carrageenan in rats. (6) The cotton-pellet granuloma in rats. Results showed that ligustilide could delay the latency of licking of the hind paw. And it could reduce the times of writhe response actions, and the inhibition rates were 61.21%,48.12%,43.61%. Ligustilide could distinctively inhibit the increasing permeability of abdominal cavity induced by acetic acid in mice, and the inhibition rates were 64.84%,57.85%,49.67%. Ligustilide could significantly inhibit the auricular edema induced by dimethy benzene, and the inhibition rates were 52.06%,41.51%,34.21%. Ligustilide could significantly inhibit the paw swelling caused by carrageenan in rats, and the inhibition rates were more than 42% from 1 to 3 hour. Ligustilide could significantly inhibit the cotton-pellet granuloma in rats, and the inhibition rates were 52.64%,50.32%,45.33%.3. Analgesic mechanism of ligustilide for experimental dysmenorrheaTo evaluate the therapeutic effects of ligustilide on rodent's experimental dysmenorrhea and to reveal the ligustilide's analgesic mechanism. Rodent's experimental dysmenorrhea was induced with oxytocin. A writhing test was employed to evaluate the analgesic effects of ligustilide, which was designed into different doses, in animal subjects. The contents of PGF2α,PGE2,TXB2,6-keto—PGF1αin the blood were observed by Elisa assay. Results showed that The ligustilide has a significant analgesic effect on experimental dysmenorrhea with dose-dependent manner. Latent period was delayed significantly, the number of writhing response times were evidently decreased as well. Besides, the content of PGE2 and PGF2αwas significantly increased, so did the TXB2, and the ratio of PGF2α/PGE2, TXB2/6-keto-PGF1αdecreased in the treated groups (P<0.01), the ratios of the model group, normal group, positive group and treated group in two doses were 0.89,0.32,0.44,0.44 and 0.36.The study ligustilide that ligustilide had an analgestic effect and can significant inhibit acute and chronic inflammation in animals. It had a significant analgesic effect on experimental dysmenorrheal, as well. Its analgesic mechanism was mediated by regulating the plasma levels of PGF2α, PGE2, TXB2 and 6-Keto-PGF1α. |
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