To Study The Effect Of Grifola Frondosa On Immunoregulation Of Cyclophosphamide Model Mice Through Regulating The Gene Expression Between Th1 And Th2 Cytokines

Objective: In order to investigate the immunoregulation of Grifola frondosa, we studied the changes of cyclophosphamide model mice Th1,Th2 type cytokines (IL-2mRNA and IL-10mRNA's expression).Methods: Kunming strain mice were randomly made up with 4 groups: normal control, model group, model treated with high dosage of Grifola frondosa , model treated with low dosage of Grifola frondosa. Other groups were treated with abdomen injection with cyclophosphamide every two days except normal control. Whether the model were successful, the group of normal control and the cyclophosphamide model were administered 0.4ml NS per mice everyday; The other groups treated with different dosage of Grifola frondosa ,which were daily administered 0.4ml drug solution of 100% and 50% concentration respectively. 14 days later, the expression of IL-2mRNA and IL-10mRNA were measured by FQ-PCR. Count the thymus index, spleen index and leukocyte of peripheral blood that data processing were determinate by one-way ANOVA method.Results: Cyclophosphamide models were developed successfully, The levels of IL-2mRNA and IL-10mRNA's expression were decreased very much obviously in model group (P<0.01 or P<0.05), compared with that in control group. The levels of the expression on IL-2mRNA and IL-10mRNA were increased very much significantly in high dosage group of Grifola frondosa (P<0.01 or P<0.05), compared with that in model group. The levels of the expression on IL-2mRNA and IL-10mRNA in serum with low dosage of Grifola frondosa were increased obviously (P<0.05) but the levels of leukocyte of peripheral blood count was no marked (P>0.05) in comparison with that in model group. Also, these was conspicuity difference in levels of expression of IL-2mRNA, spleen index and leukocyte of peripheral blood count in serum between high and low dosage group of Grifola frondosa (P<0.01 or P<0.05).Conclusion: Grifola frondosa can enhance significantly levels of IL-2mRNA and IL-10mRNA's expression serum with cyclophosphamide model mice.