Effect And Mechanism Of Ginkgo Biloba Extract On Renal Tubular Epithelial-Mesenchymal-Transition In Type 1 Diabetes Rats

Objective To explore the effects of Ginkgo Biloba extract on protecting renal tubular epithelial cell from oxidative stress in diabetic rats,and to explore its inhibition of renal tubular epithelial–mesenchymal-transition and Renal Interstitial Fibrosis.Methods The diabetic rat model was established by single intraperitoneal injection of STZ. Various doses of GBE were given to animal subjects by intraperitoneal injection every day. FBG, Scr, BUN, 24-hour urine protein, SODand MDA were measured after 8 weeks from initial intervention. The histological structure of kidney were observed by using HE,PAS and MASSON staining. The expression feature of proteinα-SMA was showed with immunohistochemistry, and Western blot was used to analyze the expression of protein Col I.Results 1. Compared with normal group, the subjects in control group had increased FBG, Scr, BUN, 24-hour urine protein with significance(P<0.01), indicating that the establishment of model was satisfactory. Compared with control group, Scr, BUN and 24-hour urine protein have significantly decreased in low dose of GBE group , however, FBG shows no significance between control group and low dose of GBE group. As for midium and high dose of GBE group, significant differences could be observed on FBG, Scr, BUN and 24-hour urine protein when it is compared with control group(P<0.01). 2.Compared with normal group, the activity of SOD in model group's renal tissue was reduced significantly(P<0.05),when the level of MDA was increased significantly(P<0.05). The activity of SOD in low dose of GBE group has significant rised(P<0.05), and the significance went greater in medium and high dose of GBE group. As for the level of MDA, the significance could be observed between model group and low, medium dose of GBE group(P<0.05),and it went greater when high dose of GBE was compared with model group. 3. Inflammatory cells infiltration, tubular vacuolization and tubulointerstitial fibrosis could be seen in model group, and its index of renal tubular-interstitial injury and realative Square meter of renal tubulointerstitium elevated significantly (P<0.01)when it was compared with normal group. renal tubular-interstitial injury and realative Square meter of renal tubulointerstitium in midium and high dose of GBE significantly released(P<0.01).4.The expression of a-SMA was significantly suppressed among subjects in model group(P<0.01).Compared with model group, the expression ofα-SMA in low dose of GBE group surpressed (P<0.05),the expression ofα-SMA in medium and high dose of GBE group was significantly suppressed(P<0.01).5. The expression of protein Col I increased with great significance in model group when it was compared with normal group. Compared with model group, the expression of protein Col I in low dose of GBE group significantly surpressed(P<0.05),and the expression went lower in medium and high GBE group.Conclusion1. GBE was capable of lowering blood glucose and Scr, BUN, 24-hour urine protein.2. Oxidative stress is exists in diabetic rats. GBE was capable of lowering the level of MDA and improve the activity of SOD to resist oxidative stress.3. GBE can improve validly renal interstitial fibrosis by impeding the progress of EMT and decreasing ECM.