The Experimental Study On The Anti-Acute Myocardial Ischemia Effect Of Four Aromatic Resuscitation Drugs

Objective:Observing parallely the Anti-Acute Myocardial Ischemia effect of four aromatic resuscitation drugs (musk, borneol, storax and benzoin), and probing into part of its mechanism of action, and compare it's similarities and differences on the role of "four character", in order to Preliminary clarify the correlation between effects of resuscitation and awakening of those kinds of drugs and th anti-myocardial ischemia, so show this kinds of drug property system of biological characterization, with the propose to provide a scientific basis for clinical application.Methods:(1) by the models of inject iso leads to myocardial, hypobaric hypoxia and specific myocardial hypoxia resulted from iso praline by the mice models of injecting iso +norm obaric hypoxia, sodium nitrite, decompression hypoxia, ligatting rat left coronary artery descending branch 24 hour were used to observe it's survival time, oxygen consumption, and model rat's myocardial infarct size, the myocardial Pathological changes and so on, to evaluate the anti-acute myocardial ischemia effect of four aromatic resuscitation drugs.(2)Use the rat model of acute myocardial ischemia to observe four aromatic resuscitation drugs defect on serum CK,LDH,AST Vitality, NO contents, Cardiac muscle of SOD,MDA contents, Na+-K+-ATPase, Ca++-Mg++-ATPase vitality, TNF-α,IL-1βlevel, to probe it's anti-ischemic mechanism.(3)used the AMI model to observe aromatic resuscitation drugs effects on model rats of awaken time, weight, body temperature, ECG, heart rates and so on,at the same time, detect models animal brain's MDA contents, Na+-K+-ATPase, Ca++-Mg++-ATPase, SOD vitality, Preliminary study four drugs relationship between drugs character's differences and effect, and relationship between "Heart" and "brain". Results:(1)four aromatic resuscitation drugs and part of extracts with anti-hypoxia effect, Iso proterenol hydrochloride induced myocardial ischemia result indicates that Moschus can remarkably reduces during 5 minutes and death oxygen consumption capacity while the mold Success, Moschus also can significantly prolonged the survival time of mice Sodium nitrite induced acute poisoning experiment; Borneolum remarkably reduces during 20 minutes oxygen consumption capacity while the mold Success, it also can significantly prolong the survival time after acute myocardial ischemia and decompression hypoxia; Styrax, petroleum ether and butanol extracts remarkably reduces during 5 minutes oxygen consumption capacity while the mold Success; Benzoinum water extract remarkably reduces during 5 minutes oxygen consumption capacity while the mold Success.(2)after AMI operation, the model rats ECG ST segment,MIS and heart rate significantly increased, it shows model is successful. (3) Compared with the AMI model, Musk, borneol, Styrax Can reduce the ECG ST segment elevation, Reduced myocardial infarct size after ischemia, Reduce the pathological damage, Reduce serum LDH, NO and myocardial MDA level, Musk, Styrax can increase the myocardial SOD, Ca++-Mg++-ATPase activity, reduced myocardial TNF-α, IL-1βcontent; Styrax, borneol can improve the myocardial Na+-K+-ATPase activity, Styrax also decreased serum CK activity.(4)Compared with the AMI model, Musk, Styrax can significantly shorten the awaken time after surgery in rats, increased body temperature, weight loss, Musk, borneol, Styrax significantly reduced the postoperative heart rate, after AMI,Styrax, borneol significantly against the brain tissue of rats with the increase of MDA content, But in brain tissue SOD, Ca++-Mg++-ATPase, Na+-K+-ATPase activity had no significant effect.Conclusion:(1)Aromatic resuscitation drug can Prolong the survival time and reduced oxygen consumption of mice with hypoxia, shows that it has anti-hypoxic effect. (2) aromatic resuscitation drugs shows myocardial protection on LAD ligation model of ischemia induce AMI, Styrax and musk is superior, Borneol second, benzoin is weak, The mechanism may be reduction the leakage of enzymes, anti-free radical damage, inhibition inflammatory response and improvement the role of energy metabolism, and other relevant links.(3) To model rats, Borneol, musk, Styrax benzoin has the same trend and common on role of myocardial ischemia, probably this kind of drug "bitter-floating-heart" drug property is one of characterization of Pharmacodynamics.(4)On model rats, borneol and musk, Styrax affecting energy metabolism, the role of some physiological indicators of the existence of some differences, suggesting that borneol was" cold".(5)AMI LAD ligation induced model rat of brain tissue MDA content was significantly increased, suggesting myocardial injury by free radical pathway may affect brain function, heart and brain revealed between the initial existence of a certain contact.