Preliminary Study On Phenolic Components And Serum Pharmacochemistry Of Herba Erigerontis Extract

Erigeron breviscapus, family Compositae, was used to treat sequelae of apoplexy by the minority nationality (e.g. the Miao) all through the ages. In recent years, researches widened it use in ophthalmic disease and indicated that its mechanism might be neuroprotection. At present, Improving blood rheology, distending the blood-vessel, reducing vascular resistance, Increasing blood flow, improving microcirculation of the optic nerve and retinal ganglion cells axoplasmic flow are mainly studied on Erigeron breviscapus. However, the effective components of optic neuroprotection are not yet clear and the study to illuminate the bioactive ingredients for treating ocular diseases in Erigeron breviscapus should be carried out in vivo and in vitro.Objective:To study the active components in Herba Erigerontis extract (HEE) for optic neuroprotection by serum pharmacochemistry method. Find a method of the qulity control of HEE.Methods:1. To establish the chromatographic method and identify the main constituents in HEE by HPLC-DAD.2. To establish the analysis method of serum pharmacochemistry for HEE by optimizing the established chromatographic conditions of HPLC-DAD, and analyse the constituents in the rat plasma after oral administration of HEE.3. To analyse the constituents in rat urine and feces after oral administration of HEE by HPLC-DAD, and compare with serum.4. To establish quality control method for the effective fraction of HEE based on the above analyses.Result:1.The main chemical constituents of HEE contained flavonoids (approximately 54.04%), such as scutellarin, apigenin-7-O-glucuronide, scutellarein, apigenin, etc., and caffeoylquinic acid (approximately 45.96%), including 6'-O-caffeoylerigoside, 3,5-dicaffeoylquinic acid (3,5-DCQA),3,4-dicaffeoylquinic acid (3,4-DCQA), etc.2. Twenty-five components were observed in rat plasma after oral administration of Erigeron breviscapus decoction, twenty-two components were observed in rat plasma after oral administration of HEE. Four original form compounds and three of the metabolites were assayed with reference standards by their retention time and ultraviolet spectrum. Most of these identified compounds were reported to have effect on optic nerve diseases, which may represent the effective substances of HEE.3. Nineteen components were observed in rat urine after oral dministration of HEE and twenty components were observed in the rat feces after oral dministration of HEE. Seven components were both detected in rat plasma, urine and feces,5 of which are scopoletin, scutellarin, apigenin-7-O-glucuronide, scutellarein, apigenin.4.The quality control for flavonoids, scutellarin,3,5-DCQA and 3,4-DCQA in HEE was established. The average recoveries of flavonoids were 101.1% (RSD=2.66%) and the average recoveries for scutellarin,3,5-DCQA and 3,4-DCQA were 97.55%～102.94%(RSD=1.58%).Conclusion:1. The compounds absorbed into blood and its metabolites are capable of being the effective constituents, and its serum pharmacochemistry should be subject to thorough investigation so as to illuminate the pharmacology and active mechanism of HEE.2. Established quality control method is simple, reliable, accurate, and can provide a reference for quality control of HEE.