The Research For Relations Between The IFN -γ ,IL-4 In Plasma And Intestinal Flora B/E Value In Children With HSP

Objective: Hench-schonlein purpura (HSP) is the common symptom of capillary allergic disease in children period, its incidence increasing year by year, Clinically in an thrombocytopenic purpura, arthritis or joint pain, abdominal pain, gastrointestinal bleeding and nephritis as the main performance, at present the pathogenesis unclear. But recent research and clinical studies show that its exist obvious immune function disorder, Cytokines role in its pathogenesis fetching attention. IFN–γand IL - 4 is the generated representative of cytokines that antigen CD4 + T cells stimulate, This study aims to examine the serum levels of them in the pathogenesis of schonlein-henoch purpura role by ELISA. in order to investigate the concentration change of HSP. Simultaneous determination of children allergic purpura acute episode and recovery in fecal bifidobacteria and bifidobacteri-a/E.coli(B/E) DNA concentration, from the Angle of micro ecology microbiome disorders and explains the relationship between children with HSP; For future application of probiotics mold preparation prevention provide theoretical basis for children allergic diease.Method: Acquisition 2010 October - 2011 February from the pediatric department in the Third Hospital Of Hebei Medical University about children with HSP 37 examples (group A) , association with the diagnostic criteria of the United States rheumatism of HSP in 1990 . Children are new-onset of sick , without apparent infection symptoms and signs, do not take any prior to admission treatments, routine urine saw no exception; A group divide to A1group(the acute phase) and A2 group(the recovery phase), and serum levels in 28 examples(group B) of normal children to healthscreening. Two groups of sex, age, there are no significant difference (P > 0.05)(see table 1). This experiment is divided into two parts: 1. Using enzyme-linked Immunosorbent adsorption Linked Immunosorbent Assay (helicase, ELISA) test each children plasma IFN–γ/ IL - 4. 2. Using fluorescent Quantitative Polymerase Chain Reaction (Flurogenic Quantitative Polymerase hypotheses, FQ - PCR) areas each children technical inspection of colon bacillus and e. coli stool DNA concentration and calculation B/E ratio; Using data from the statistical analysis software SPSS 13.0 statistical processing. With X_±S determination results, said statistics processing with two sample mean differences, if the t test, the variance not neat t 'inspection. In p <0.05 as inspection standards.Result:1 Comparision of quantity of fecal microorganisms in each groups1.1 A group compared with B group: The quantity of bifidobacteria,E.coli and B/E in A group is lower than that in B group, the difference was statistically significant (P <0.05) (see Table 2).1.2 A1 group compared with A2 group: The quantity of bifidobacteria in A1 group is higher than that in A2 group, the difference was statistically significant (P <0.05); The quantity of E.coli in A1 group is higher than that in A2 group, the difference was statistically significant (P <0.05) ;B/E value between A1 and A2 group was not statistically significant (P> 0.05);(see Table 3).2 The different level of IFN–γ, IL- 4 (pg/ml) in each group2.1 A group compared with B group: The IFN–γlevel in plasma in A1 was respectively lower than those in B group, the difference was statistically significant (P <0.05). The IL- 4 level in plasma in A was respectively higher than those in B group, the difference was statistically significant (P <0.05) (see Table 4).2.2 A1group compared with A2 group: .The IFN–γlevel in plasma in A1 and A2 group were not different (P> 0.05). The plasma of IL- 4 in A1 was respectively higher than those in A2 group, the difference was statistically significant (P <0.05) (see Table 4). 3 The level of IFN -γ/IL -4 in HSP3.1 The level of IFN -γ/IL -4 in A is lower than B group, the difference was statistically significant (P <0.05) (see Table 4).3.2 The level of IFN -γ/IL -4 in A1 is lower than A2 group, the difference was statistically significant (P <0.05) (see Table 5).4 The correlation analysis between B/E and IFN -γ/IL -4 in plasma in children with HSP showed a positive correlation in A1 group (r=0.770, P<0.05) (see Fig.5 ).Conclusion:1 The intestinal flora of children with HSP at the acute phase attack diordered, manifested as reduction in the quantity of bifidobacteria and B/E. With clinical treatment and the improvement of symptoms, the amount of bifidobacterium gradually increased, and the amount of bifidobacterium increased faster than that of E.coli, but there was still intestinal dysbacteriosis during the recovery stage of HSP.2 The cytokines type lymphocyte subsets express abnormalities about Th1 / Th2 in HSP, mainly display the reduce in the serum of IFN-γand the increasing in the serum of IL-4, display the disordered in Th1/Th2 at theacute phase, manifested Th1/Th2 participate the mechanism of HSP. 3 With B/E represents the intestinal flora , with Th1 / Th2 represents the auxiliary T cell function, the disorder about B/E value and Th1/ Th2 in HSP, B/E value and Th1/ Th2 show a positive correlation in them, manifested the disorder of intestinal flora and immune function,which participate the pathology process,and the gastrointestinal tract flora keep the function of organism.4 Our investigation can provid an significant theoretical basis for probiotics preservation and treatment HSP through adjunction probiotics.