The Study Of Repairing Long Bone Defects In Rabbits With The Activity Of Blood Vessel Endothelial Cells On Inorganic Active Elements Of Bone Scaffold

PurposeBone tissue engineering in recent years there are many kinds of new bone tissue engineering scaffold material of bone defect repair methods, such as tissue engineering scaffold material of Growth Factor and tissue engineering scaffold materials futures vascular pedicle muscle flaps, etc. . In this study, the joint bone tissue engineering scaffold material used in vascular endothelial cells of bone defects, tissue engineering of bone cells observed composite materials used in bone repair cells to differentiate into bone and bone status of angiogenesis. Experimental research methods intended to better explain the proliferation of vascular tissue engineering of bone regeneration and promote the mechanism of bone.MethodsThe enzyme digestion, extraction of rabbit's own long bone marrow, isolate, culture expansion of bone marrow stromal cells to the third generation, joined the conditioned medium after the induction of differentiation into vascular endothelial cells, culture and passage were inoculated in inorganic active elements of the scaffolds to form Tissue engineering of bone cell materials composite, ported to the artificial femur defects on composite tissue engineering of bone cells transplanted as the experimental group, the simple transfer of inorganic materials as active elements stent control group. By using immunohistochemical staining, after X-quantitative detection method for tracking the tissue engineering of bone model, the bone defect the local expression of bone mass and changes in VEGF was observed after the repair of femur bone and vascular regeneration, and the results were compared.ResultsRegularly observed after bilateral bone osteoblasts were seen dense bone tissue repair, angiogenesis reconstruction; Immunohistochemistry showed that the experimental group of early osteogenesis and angiogenesis speed speed than the control group, X ray detection experiment also shows group of bone defects after year and the external callus higher level of X radiopacity. Show that the terms of the experimental group and the blood vessels in the bone regeneration compared with the control group had certain advantages.ConclusionsActivity induced by inorganic elements of bone scaffolds in repairing bone defects when used effectively promote bone cell growth, differentiation and enhance its osteogenic characteristics, and cells combined with tissue engineering scaffold material for bone repair in the repair of bone defects and improve the inorganic activity induced bone scaffold elements to differentiate into bone and blood vessels in the regeneration of the advantages, so that the repair and reconstruction of bone defects effectively improve efficiency.