| Objective To investigate the influence of nano-hydroxyapatite materials (Nano- hydroxyapatite, NHA) with canine vein endothelial cells physiological function, and the surface endothelialization after implant in body . To provides an ideal scaffold material for the application of tissue engineering mechanical valves.Methods Part I: canine vein endothelial cells were subcultured, one group co-cultured of nanometer hydroxyapatite materials in vitro, the other group did not add material as control group vaccinate cultivate. Collect the culture fluid 1,3,5,7 day respectively, enzyme-linked immunosorbent assay PGI2 levels in order to know NHA materials influence the physiological functions of canine vein endothelial cells . Part II: Nanometer hydroxyapatite materials and artificial mechanical heart valve leaf materials were implanted in dogs right atrium, scanning electron microscopy to understand the endothelial situation of the materials surface.Results (1)Acquisition the canine blood vessel cells and cultivate them, though immunofluorescence, there be verified that these cells were canine vein endothelial cells. canine vein endothelial cells have a good growth, adhesion, and proliferation on nanometer hydroxyapatite materials . detection of PGI2 content with the blank group, there were no significant differences. (P>0.05) (2) Nanometer hydroxyapatite and normal valve materials were implanted in the right atrium of dogs eight weeks, take out of these materials and wash them. Scanning Electron Microscope observation affirmed the surface of NHA have endothelial cells layer. Artificial mechanical heart valve leafs surface were not see endothelial cell layer, and a small amount of thrombus attached.Conclusion Nanometer hydroxyapatite materials of vascular endothelial cells had no effect on the physiological functions, NHA has a good cell/tissue compatibility, implantation in vivo of dogs has good endothelialization, can become scaffold material source of tissue engineered heart valve.
|
PDF Full Text Request