Exhaled Breath Condensate Cysteinyl Leukotrienes And 8-isoprostane In Asthmatic Children

OBJECTIVE: The aim of our study was to analyze the change of Cysteinyl Leukotriene (Cys-LTs) levels and 8- Isoprostane (8-iso-PG) levels in the exhaled breath condensate (EBC) of asthmatic children from acute exacerbation to clinical remission, and investigate the role of the detection of Cys-LTs and 8-iso-PG in of EBC in the diagnosis of asthma and its severity and pathogenesis, and explore the relationship between the lCys-LTs level 8-iso-PG through measuring Cys-LTs levels and 8-iso-PG in the EBC of asthmatic children.METHODS: The outpatient or inpatient asthmatic children of the pediatrics in the First Affiliated Hospital of Anhui Medical University from October 2010 to December 2010 and a group of healthy children were studied. All subjects'EBC was collected by the RTube produced by American Respiratory Research. The concentration of Cys-LTs and 8-iso-PG in EBC were measured by enzyme-linked immunosorbent (ELISA) produced by Groundwork Biotechnology Diagnosticate, and compared among children in asthmatic exacerbation, asthmatic remission, and healthy condition. And the relevance of their change would be explored at the same time.RESULTS: (1)Cys-LTs levels in EBC were higher in asthma exacerbation, compared to healthy controls (respectively 68.32±3.38 pg/L vs 63.51±3.83 pg/L, P<0.05) while no significant difference were found between asthmatic remission and asthmatic exacerbation (respectively 68.32±3.38 pg/L vs 66.87±3.49 pg/L,P< 0.05)or healthy controls. (2)8-iso-PG levels is higher in asthmatic exacerbation compared to asthmatic remission (79.07±5.20 pg/L vs 65.91±3.62 pg/ L,P<0.05). Moreover, the 8-iso-PG levels were significantly higher in asthmatic remission than in healthy controls (54.56±3.83 pg/L). (3)Through the relevance analysis of the Cys-LTs and 8-iso-PG levels in EBC among the three groups, Cys-LTs levels in EBC of asthmatic exacerbation significantly correlated with 8-iso-PG levels (n1=35,r1=0.61,P<0.05) while no significant correlation between 8-iso-PG levels and Cys-LTs levels in asthmatic remission.CONCLUSIONS: the increase of 8-iso-PG levels in EBC of bronchial asthmatic patients correlates with the disease and its control. Therefore, 8-iso-PG can be an objective indicator for asthmatic diagnosis and healing efficacy. Cy-LTs levels increase in the EBC of bronchial asthmatic according to disease severity. The two levels correlate during asthmatic exacerbation, indicating that a link between airway oxidative stress and inflammation among asthmatics.