Correlation Analysis Of Meiotic Recombination Frequencies With Age And Synaptonemal Complex Length In Human Spermatocyte

Background Aneuploidy, a numerically chromosomal abnormality,is a major cause of infertility,spontaneous abortion and birth with defects.The vast majority of frequently observed aneuploidy in human resulted from non-disjunction of homologous chromosomes in meiosis I. Molecular genetics study found that approximately 50%,80%and 100% in 47,XXY,45,X,and 47,XYY caused by non-disjunction of homologous chromosomes in meiosis I from paternal. Directly carried out on sperm chromosome studies have shown about 7.64% of the human sperm aneuploidy. Understanding of the mechanism of sperm aneuploidy in meiosis is very important to promote male reproductive health and improve the quality of the population.Objective To understand the degree of difference recombination frequency of fertile males , correlation between meiotic recombination frequencies and age in human spermatocyte and the recombination sites are distributed in the synaptonemal complex features,this paper studies meiosisΙof fertile male meiosisΙand determined recombination frequency and autosomal synaptonemal complex length between individuals.Materials and Methods 6 cases from the surgical castration elderly men suffering prostate cancer and 4 cases from testicular cancer patient after orchiectomying. Histopathological analysis confirmed that the process of normal spermatogenesis. Prepared by spreading synaptonemal complex combination of immunofluorescence staining and Using antibodies against SCP3 (marks lateral elements of the SC), MLH1( Mark recombination foci), and CREST (Marker centromere protein),we measure the process of meiosisΙ, respectively, each period cell ratio,pachytene spermatocytes homologous recombination frequency and the total autosomal SC length and analysis correlation betweenmeiotic recombination frequencies and age in human spermatocyte and relationship recombination sites and synaptonemal complex length complex length.Results Through analysis of 10 cases of male fertility the process of meiosisΙ,we find that Cell ratio of leptonema, zygotene, and pachytene is no significant difference between individuals(p>0.05),recombination sites per cell average 49.4±4.4, range 33 to 63, with significant individual differences and only 0.4% (1 / 220) of the SC lack of MLH1 sites on the autosomal. All 10 cases of male autosomal SC length measurements find that the average SC length of each range of 354μm ~ 385μm and with recombination sites positive correlation. Analysis between age and the frequencies of recombination foci fail to get any significant statistics correltation, suggesting aging contribute nothing to the variation among individuals.CONCLUDE Each stage cell ratio in meiosisΙof fertile male and recombination frequency are related to age. Recombination foci significant differences among individual and the length of SC on chromosome have a positive correlation with recombination sites foci...