| Objective: To investigate the associations between two polymorphisms in pre-miRNA with hepatocellular carcinoma (HCC) susceptibility in Chinese population. Methods: A case-control study with 963 HCC cases and 829 HBsAg positive controls and 852 HBsAg negative controls was conducted, and the genotypes were determined by the Primer Introduced Restriction Analysis-PCR (PIRA-PCR) assay.Logistic regression analysis was used to evaluate the odd ratios (ORs) and 95% confidence intervals (CIs) of hsa-mir-146a rs2910164 C>G and hsa-mir-196-a2 rs11614913 T>C with susceptibility of HCC. Results: The genotype frequencies of CC, CG and GG of rs2910164 were 34.5%(319/925), 48.6%(450/925) and 16.9(156/925) in cases, 36.4%(274/753), 45.0%(339/753) and 18.6%(140/753) in HBsAg positive controls, 36.1%(303/840), 46.0%(386/840) and 18.0%(151/840) in HBsAg negative controls. The genotype frequencies of TT, CT and CC of rs11614913 were 29.7%(277/934), 48.1%(449/934) and 22.3%(208/934) in cases, 30.3%(238/785), 51.0%(400/785) and 18.7%(147/785) in HBsAg positive controls, 28.6%(239/837),49.8%(417/837) and 21.6%(181/837) in HBsAg negative controls. No significant associations was observed between these two SNPs and HCC risk after adjusting by age, sex, smoking and drinking status (compared with HBsAg positive controls: hsa-mir-146a rs2910164: adjusted OR= 1.10, 95% CI=0.90-1.36 (CG/GG vs CC); hsa-mir-196-a2 rs11614913: adjusted OR=1.01, 95% CI=0.81-1.25 (CC/CT vs TT); compared with HBsAg negative controls: hsa-mir-146a rs2910164: adjusted OR=1.06, 95% CI=0.87-1.29 (CG/GG vs CC); hsa-mir-196-a2 rs11614913: adjusted OR=0.94, 95% CI=0.76-1.16 (CC/CT vs TT)). No significant associations were observed between these two SNPs and HCC risk in the subgroups stratified by age, sex, smoking and drinking status. Conclusion: hsa-mir-146a rs2910164 C>G and hsa-mir-196-a2 rs11614913 T>C may not play an important role in the susceptibility of HCC in Chinese.
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