| Background and Objective: Slit family is a important role in the regulation of neural stem cells migration, positioning and directional growth of axons.In recent years some studies discovered, slit2 guide embryonic central and peripheral nervous system neuronal migration and axonal growth, but also involved the neurological function recovery and reconstruction of neural networks when brain injury in adults. This study focused on the expression changes of Slit2, Robo2 and RhoA in the infarcted cortex and hippocampus after cerebral ischemia in adult rats from each group. In addition, Observed proliferation of new nerve cells in anterior subventricular zone (SVZ).This study mainly to investigate the effect of repetitive transcranial magnetie stilnulation (rTMS) on the infarcted cortex expression of Slit2 and Robo2 at the acute stage of middle cerebral artery occlusion injury. we explored the possible effect of rTMS on the neurological function recovery of the brain.Methods:Partâ… :The focal middle cerebral artery occlusion model was induced by Craniotomy Electrocoagulation in rats. Sixty adult male Sprague-Dawley(SD) rats were selected and randomly divided into 5 groups including the sham operation group and group after left middle cerebral artery occlusion (MCAO) with 1d,3d,7d,14d and 1m. In the corresponding time points,the rats were killed to measure the protein including Slit2, Robo2,RhoA,BrdU and Nestin by Immunohistochemistry or Western blotting in the infarcted cortex and hippocampus.Partâ…¡:Fifty-four adult male SD rats were selected and randomly divided into 3 groups including sham-rTMS,high-frequency low-intensity rTMS,high-frequency high-intensity rTMS group after left MCAO. Continuous stimulation rats were killed to measure the protein including Slit2, Robo2, BrdU and Nestin by Immunohistochemistry or Western blotting after 1weak and 2weak. Comparison the neurological score (NSS) among the groups at the same time point explored the effect of rTMS on functional recovery after cerebral ischemia.Results:Partâ… :There were the neural guidance factor Slit2 and Robo2 expression in the infarcted cortex and hippocampus at different time points on MCAO. There were RhoA,Brdu and Nestin positive cells exist. Similarly, its receptor Robo2 expression reached a peak at 7 days after ischemia, just as the proliferation peak of neonatal nerve cells.Partâ…¡: High-frequency rTMS group symptoms of nerve injury significantly improved compared to sham-rTMS group. The rTMS treatment interventions increased expression of Slit2/ Robo2 expression in the infarcted cortex (p<0.05). High-frequency rTMS treatment Significant increased the number of Brdu and Nestin positive cells in the cerebral ischemia cortex than sham-rTMS group (p<0.05).Conclusion: After focal cerebral ischemia in rats,the neural guidance factor Slit2 participated nerve cells repair in the central nervous system. So rTMS could promote the secretion of neuronal guidance factors, and increase the number of newborn neurons in the cerebral cortex, improve newborn nerve cell proliferation, migration, and the rehabilitation of neurological.
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