Prepared Microcapsule With Human Retinal Pigment Epithelial Cell By High Voltage Electrostatic System And Protecting Microencapsulated Cells Were Researched

Cell microencapsulation is a kind of effective method which avoidsimmune rejection so as to improve the survival rate of transplanted cells.Human Retinal Pigment Epithelial Cell (hRPE) has a huge potential in celltransplantation about Parkinson disease because of its Tyrosine hydroxyl ofenzyme activity and the dopamine. This research explores the optimizatingcondition for the microcapsule preparation and tries to prepare themicrocapsule with hRPE cells. We expect that the microcapsuled hRPE cellinjury during the preparation can be reduced by some cytokines and cellprotective substances.Prepared Alginate-Chitosan-Alginate (ACA) microcapsule with hRPEcells by using our self-made high voltage electrostatic system, we canobserve the effects of every factor to microcapsule based on the otherfactors unchanged. These factors include physical and chemical factorssuch as woltage, injection rate, electrode distance, needle inner diameterand sodium alginate and chitosan solution concentration, film reaction timeand so on. Microcapsule morphology was observed by inverted opticalmicroscope, its diameter done by cell counter and its stability testifiedindirectly. The best factors are electrode distance with 30mm, needle innerdiameter with 0.292mm, electric voltage with 7kv, injection rate with20mL·h^-1,sodium alginate and chitosan solution concentration with 15g·Land film reaction time with 15minutes.The cell growth could be harmed inevitably by microencapsulationand changing environments. In order to improve the cell's growth condition,we observed initially the effects of fructose , fibroblast growth supplement (FGS) and taurine on the total number, the survival rate and the survivalnumber of Human Retinal Pigment Epithelial(hRPE)Cell which areencapulsed on the basis of preparing the cell microcapsule.Microcapsule with hRPE cells were inoculated into four kinds ofculture mediums respectively which contained nothing, fructose, FGS andtaurine. Then observe the total number, the survival rate and the survivalnumber of the microencapsulated hRPE cell on the 0, 1, 3, 7 days. The cellnumber is counted by cell counter. The survival rate of the cells was testtedby trypan blue staining.The diameter of ACA microcapsule of hRPE is 450μ±13μm diametercan be gained. After 7days, four groups of hRPE cells microencapsulatedsurivial rate is (75±16.8)% at least. But they have no statistically significantdifferences(P>0.05); the total number of blank group, fructose group,taurine group and fibroblast growth supplement group is(6.1±0.6)×10⁴,(6.0±0.5)×10⁴,(7.2±0.4)×10⁴ and (8.0±0.5)×10⁴ , taurine group andfibroblast growth supplement group has significant increased (P<0.05).Four groups'survival number of the cells has significant difference just astheir total number (P<0.05). The total number of cells and the survivalnumber of microencapsulated hRPE cells microencapsulated into culturemediums which contained FGS and taurine can be increased within 7 days.