Empirical Study Of Treatment Of Lung Cancer By Ultrosound Microbubble Uniting CD-UPRT/5-FC Suicide Gene In Nude Mice

Objective: To investigate the treatment of lung cancer by ultrosound microbubble uniting CD-UPRT/5-FC suicide gene in nude mouseMethords: To establish nude mice model with A549 cell ,after 2w the volunm of transplantation tumor of nude mice can be (0.5±0.11)cm3.dividing them into 4 groups,A group is control group,B group injected ultrosound microbubble into tumors ,C group injected suicide gene and 5-FC,D group injected microbubble suiciede gene and 5-FC.Taking notes of the diameter Every 4 day,accont the volum of tumors. hematoxylin and eosin stain(HE stain) and Examing CD protain by immunohistochemisty after 20days.Result:1.analysis of variance:the volum between A and B has no statistical significance(P>0.05), the volum between C and A has statistical significance(P<0.05), the volum between D and A has statistical significance(P<0.05), the volum between C and D has statistical significance(P<0.05). 2.HE stain:In A and B group,the amount of cells is large,cells grow tight Cell nucleus is big and deep stain.In C and D group 3. immunohistochemisty:A grouop and B grouop have no CD protain,C group and D group have CD protain,the protain of D group is more than that of C group,analysis of IOD between C group and D group has statistical significance(P<0.05).conclusion:ultrosound microbubble can increase the cell-transfection efficiency of CD-UPRT suicide gene,then inhibite the growth of lung cancer in nude mice.