Influence And Related Study Of Diammonium Glycyrrhizinate On Hepatic In Brain Death Rats

Part I Establishment and related study of brain death model for ratsObjective To establish the brain death model in rats by increasing intracranial pressure (ICP) and to compare explosive with gradual brain death induction under ICP.Methods A total of 60 rats were randomized to control group (Group A) with 20 rats and brain death group with 40 rats which was bisected explosive brain death (Group B) and gradual brain death (Group C).Supporting by respirator, brain death state was maintained for 6 h.The system functions of biological monitor the change of arterial pressure and heart rate in the experiment process, then we compared the rate of success.Results Brain death model was inducted successfully in brain death group (32 rats).Arterial pressure and heart rate were increased sharply at the end of the induction, and then decreased by degrees. In group B, the arterial pressure and heart rate decreased sharply and obviously, and there were five rats removed from the research because of their too low arterial pressure. But in group C, the arterial pressure and heart rate decreased slowly. Compare with the arterial pressure and heart rate of group A, the peak and trough of brain death group's had significant difference(P<0.05). The success rate of group B was 70%(14/20),which less than 90%(18/20) of group C.Conclusion The stable and reliable model of brain death in rats could be established by increasing ICP, and the gradual brain death induction under ICP had a higher rate of success. PartⅡRelated study of brain death on hepatic injury in ratsObjective To establish the brain death model in rats by increasing intracranial pressure (ICP) and to investigate the influence on hepatic injury in brain death rats.Methods A total of 40 rats were randomly bisected into control group and brain death group,the brain death group was inducted by gradual brain death induction under ICP, Supporting by respirator, blood and liver specimen were harvested at 0,2,4 and 6 hours after brain death induction.the levels of ALT and AST in blood were determined by automatic biochemical analyzer,the sample of liver were collected for histopathology,the levels of TNF-αand MHC-Ⅱin liver specimen were determined by immunol histochemistry. The same treatment was carry out in control group.Results In brain death group, the levels of ALT,AST, TNF-αand MHC-Ⅱwere higher than the levels in control group. After brain death induction, the degeneration of liver cells were found in histopathology and became obviously in 6h group. Compare with the same time of control group, the levels of brain death group had significant difference(P<0.05).Conclusion The brain death could cause damage to hepatic in rats,and also increase the levels of TNF-αand MHC-Ⅱ.the brain death induction sustained longer and the extent of damage became more severity. PartⅢProtective effect and related study of diammonium glycyrrhizinate on hepatic in brain death ratsObjective To establish the brain death model in rats by increasing intracranial pressure (ICP),after brain death induction, diammonium glycyrrhizinate(DG) were infused to investigate the protective effect on hepatic in brain death rats.Methods A total of 25 rats were randomized to control group (Group A) with 5 rats, brain death group (Group B) with 5 rats and DG group (Group C) with 15 rats. Group B and C were inducted by gradual brain death induction under ICP, Group C was divided into 3 groups:low concentration group (10mg/kg), medium concentration group (20mg/kg) and high concentration group (30mg/kg). In group C, DG was pump in femoral vein after brain death induction. Supporting by respirator, blood and liver specimen were harvested at 4 hours after brain death induction.the levels of ALT and AST in blood were determined by automatic biochemical analyzer,the sample of liver were collected for histopathology,the levels of TNF-αand MHC-Ⅱin liver specimen were determined by immunol histochemistry, hepatocyte apoptosis was determined by terminal deoxynucleotidy transferase-mediated dUTP-biotin nick end labeling assay (TUNEL). The same treatment was carry out in group A and B.Results In group B and C, the levels of ALT,AST, TNF-α,MHC-Ⅱand AI were higher than the levels in group A. In group C, the levels of ALT,AST, TNF-α,MHC-Ⅱand AI were lower than the levels in group B, and particularly in medium concentration group. After brain death induction in group B and C, the degeneration of liver cells were found in histopathology. The degeneration of liver cells in group B became more serious than group C, and particularly in medium concentration group. The change during every group had significant difference(P<0.05).Conclusion The brain death could cause damage to hepatic in rats,and also increase the levels of TNF-αand MHC-Ⅱ.DG management on the brain death donor can improve the morphology and function of liver, and also decrease the levels of TNF-α, MHC-Ⅱand hepatocyte apoptosis, particularly in edium concentration group.