The Affect Of Nrf2 And γ-GCS Expression After Intervened With Matrine In The Bleomycin-induced- Pulmonary Fibrosis-rats

Objective:By investigated the chang of the expression of Nrf2 andγ-GCS in the Bleomycin-Induced Pulmonary Fibrosis rats after treated with Matrine.Study the effect and perhaps mechanism in the treatment of matrine in pulmonary fibrosis.Methods:120 adult male SD rats were randomly divided into control group (group A),experimental model group (group B),Prednisone Acetate group (group C),Matrine 50mg group(group D) and Matrine 200mg group(group E).Pulmonary fibrosis model was established by injected with Bleomycin by intratracheal.Detected the concentration of HYP in the lung tissue.The expression of Nrf2 andγ-GCS mRNA in lung tissue were measured by reverse transcription-polymerase chain reaction(RT-PCR).The protein expressions of Nrf2 andγ-GCS were measured by Western blot.Results:(1) Except group A,the else groups performance with varying degrees of alveolitis and fibrosis. Treatment groups reduced the degree of alveolitis and pulmonary fibrosis,the difference with experimental model group was significant. The reduce of high-dose matrine group was more pronounced.(2) The concentration of HYP was significantly higher in the else groups than group A.And it was the highest in the group B(P<0.01).It reached the peak at 14th (P<0.01).(3) Contrast to group A,The protein expression ofγ-GCS and Nrf2 were significantly higher in the lung tissue in the else groups. Group C,D and E were higher than Group B (P<0.01).It reached the peak at 7th(P<0.01).(4)Contrast to group A ,The expression ofγ-GCS and nrf2mRNA were significantly higher in the lung tissue in the else groups. Group C,D and E were higher than Group B (P<0.01).It reached the peak at 7th(P<0.01).(5)They were positively correlated between the expression ofγ-GCS protein and the expression of Nrf2 protein,mRNA.Conclusions:1. Matrine inhibit pulmonary fibrosis in rats induced by bleomycin.2. By controlling the expression of Nrf2 to indirectly control the expression ofγ-GCS. This is a possible mechanism why matrine inhibit pulmonary fibrosis in rats.