Assessment Of Left Ventricular Regional Myocardial Systolic Function After Bone Marrow Mesenchymal Stem Cells Injection In Rabbits With Acute Myocardial Infarction By Speckle Tracking Imaging

Objective:To assess the left ventricular regional myocardial systolic function after bone marrow mesenchymal stem cells (BMSCs) injection in rabbits with acute myocardial infarction (AMI) by two-dimensional ultrasound speckle tracking imaging. Methods:T wenty four healthy rabbits were randomly divided into three groups:group of sham-operated, group of masculine control (AMI was induced by ligation of left anterior descending coronary artery), group of cell infusion (after two weeks of AMI, bone marrow mesenchymal stem cells were injected to the region of AMI).4w after cell deliver two-dimensional strain images were acquired from LV short-axis view (at the levels of mitral annulus, muscle papillary and apex), radial strain rate (SrR), circumference strain rate (SrC), rotation rate (RotR) and rotation (Rot) of three levels in short-axis views were measured by speckle tracking imaging. The conventional echocardiography indices included left ventricular diameter of end diastole (LVEDd), left ventricular ejection fraction (LVEF), left ventricular fractional shortening (LVFS). The catheter indices included left ventricular systolic pressure (LVSP), left ventricular end diastolic pressure (LVEDP) and maximum rate of rise and descend of left ventricular pressure (LVdp/dtmax). Results:Compared with sham-operated group, rabbits had significantly larger LVEDd, lower LVEF and LVFS, lower LVSP and LVdp/dtmax, higher LVEDP in control group. SrR, SrC, RotR and Rot of left ventricular anterior regional myocardial function and global myocardial function of three levels in short-axis views were lower. Compared with control group, the rabbits had significantly smaller LVEDd, larger LVEF and LVFS, larger LVSP and LVdp/dtmax, lower LVEDP. SrR, SrC, RotR and Rot of left ventricular anterior regional myocardial function and global myocardial function of three levels in short-axis views were larger in group of cell infusion. There was no significant between group of cell infusion and group of sham-operated. Conclusions:Speckle tracking imaging can evaluate the regional myocardial systolic function of the area of bone marrow mesenchymal stem cells transplantation accurately.