General Toxicological Study On Xi Pa Yi Kui Jie'an In Experimental Animals

Objective:Kui Jie'an enema (KJA) has been used for the treatment of ulcerative colitis(UC) in Uyghur medicine. However, there is very little information about the toxicity of KJA. To provide safety information of the KJA, we evaluated its acute,sub-chronic toxicity and rectal irritation in animals.Methods:In the acute toxicity study, ICR mice were administered with KJA extract rectally at dosage of 10 g/kg body weight After a single administration, clinical signs,body weights,food and water consumption were observed for two weeks. In the sub- chronic toxicity study, KJA was administered to rats at dosage of 0,0.2,0.8 and 2 g/kg/day for 3 and 6 months.Mortalities,clinical signs,body weights,food and water consumption,hematological and biochemical parameters,organ weights and histological examination were perfomed during the study period. In the rectal irritation test, KJA was administered to rabbits at dosage of 0.38,0.04 g/kg/day for 14 days. Mortalities,body weights,food and water consumption were monitored during the study period. Results:We found no mortality and abnormalities in clinical signs,body weights,food and water consumption no obvious differences between the two groups. In the sub-chronic toxicity study, There is no significant changes in general behavior,hematological and biochemical parameters, except for MCV,MCHC T-BIL and ALT. histological examination showed that there is very few morphological alterations between the control and experimental group, but had not any statistical differences between the groups.In the rectal irritation test, there is no any morphological alterations of colon and rectum in rabbits.Conclusion:The present study indicated that KJA has no significant acute,chronic toxicity and rectal irritation in animals.