| Background and objectiveCurrent knowledge suggests immunoglobulin G (IgG) molecules can be produced by differentiated B-lymphocytes only. Recently,more and more researchers demonstrate that human cancers of epithelial origin, for example, liver carcinoma, breast cancer can also produce IgG in both cytoplasmic and secreted forms. It has been investigated that IgG could be expressed in some normal cell types. However, the expression of IgG in normal adult rat liver has never been detected. We do the study to investigate the expression of immunoglobulin G ( IgG) in the adult rat hepatocytes.Materials and methods1. Tissue samples: Liver samples were collected from adult SD rats. The male Sprague–Dawley rats weighing 250-300 g were obtained from the Laboratory Animal Center of The Shantou University Medical College.2. Immunohistochemistry: To test the expression of IgG protein in the adult rat hepatocytes.3. in situ hybridization: To test the expression of IgG mRNA in the adult rat hepatocytes.4. Laser capture microdissection (LCM) related single-cell RT-PCR: To test the expression of IgG mRNA in the adult rat hepatocytes. Results1. Immunohistochemistry: IgG immunoreactivity was observed in adult rat hepatocytes.2. In situ hybridization: IgG mRNA was observed in adult rat hepatocytes.3. Laser capture microdissection (LCM) related single-cell RT-PCR: IgG mRNA was observed in adult rat hepatocytes.ConclusionIn our study, the expression of IgG in normal adult rat liver was detected. |
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