Experimental Study On The Effect Of Tubulointerstitial Injur Of IGA Nephropathy In Rat With Shen-Yan-Zhi-Xue Pill

Objective: Through experimental study on the effect of tubulointerstitium injur of IgA nephropathy(IgAN)in rat with SHEN-YAN-ZHI-XUE pill, further discusses this sickness to have the development mechanism,for SHEN-YAN-ZHI-XUE pill to improve tubulointerstitial injury mechanism to provide a more thorough theoretical and experimental basis.Methods:The oral administration to rats with bovine serum albumin acidification of water and tail vein injection of Lipopolysaccharide and to inject hypodermically Castor Oil +CCL4complex method IgAN model, observation of IgAN SHEN-YAN-ZHI-XUE pill urine red blood cell count in rats, a 24-hour urinary protein, the kidney function, the liver function, a sensitive indicator of renal tubular function in urinary retinol-binding protein (RBP) and N-acetyl-β-D-glucosaminidase (NAG), simultaneously using the light microscope,immunofluorescence, immunohistochemistry ,RT-PCR and Motic the image 3.2 image analysis software observes the kidney organization (tubular-mesenchymal) the pathology change, the renal glomerulus are the membrane area IgA deposition situation and the Monocyte Chemoattractant Protein-19MCP-10and AngiotensinⅡreceptor type1(AT1),AngiotensinⅡreceptor type2(AT2)in tubulus renalis-mesenchymal expression situation and quantitative analysis.Results:1.SHEN-YAN-ZHI-XUE pill can reduce the red blood cells in urine and urinary protein, lower serum creatinine and blood urea nitrogen, protect the renal function, lower urinary RBP, NAG enzyme the protection of renal tubular function,the overall effect is better than the control group tripterygium(P<0.05), and does not have the Tripterygium wilfordii multi-glucoside piece to the liver function harm side effect; 2. To reduce IgA in the glomerular mesangial areas of deposition, to reduce glomerular injury; 3. To reduce the glomerular and tubulointerstitial injury pathology; 4. May reduce MCP-1 activity then ruduce the renal tissue level of AT1,AT2 and other inflammatory mediators, regulation of inflammatory cytokines and reduce inflamematory reaction of tubulointerstitial injury.Conclusion:SHEN-YAN-ZHI-XUE pill has the remarkable prevention function to the tubulointerstitium injur of IgAN, the overall curative effect surpasses the Tripterygium wilfordii multi-glucoside piece control group, for clinical further applied has provided the theory basis.