Experimental Study On The Effect Of Tubulointerstitial Injur Of IgA Nephropathy In Rat With Shen-Yan-Zhi-Xue Pill

Objietive:through experimental study on the effect of tubulointerstitium injur of IgA nephropathy in rat with SHEN-YAN-ZHI-XUE pill,further discusses this sickness to have the development mechanism to provide a more thorough theoretical and experimental basis.Methods:the oral administration to rats with bovine serum albumin acidification of water and tail vein inject of bovine serum albumin and staphylococcal enterotoxin B complex method IgAN SHEN-YAN-ZHI-XUE pill urine red blood cell in rats ,a 24-hour uri protein,the nary kidney function,the liver function,a sensitive indicator of renal tubular function in urinary retinol-binding protein(RBP)and N-acetyl-β-D-glucosaminidase(NAG),simultaneously using the light microscope,immunofluorescnce,immunohistochemistry and Motic the image 3.2 image analysis software observes the kidney organization (tubular-mesenchymal)the pathology change,the renal glomerulus are the membrane area IgAdeposition situation and the nucleus transcription annexin-1 and macrophage migration inhibiting factor(MIF) in the tubular - interstitial expression and quantitative analysis.Resulit:1. shen-yan-zhi-xue pill can reduce the red blood cells in urine and urinary protein, lower serum creatinine and blood urea nitrogen to protect renal function, lower urinary RBP, NAG enzyme to improve renal function, the overall effect is better than triptolide group (P <0.05), and no Thunder Rattan wilfordii tablet side effects of liver damage;2. Reduction in glomerular mesangial IgA deposition, reduce glomerular injury;3. Reduce the glomerular and tubulointerstitial pathological damage;4. May regulate annexin-1 and MIF of inflammatory mediators, regulation of inflammatory cytokines, reducing inflammation of the glomeruli, tubulointerstitial damage, thus improving the function of tubulointerstitial play it's therapeutic effect.Conclusion:shen-yan-zhi-xue pill on tubulointerstitial injury in IgAN significant preventive effect, the overall effect is better than the control group Tripterygium for further clinical applications of the theory.