Comparative Study Of α-lipoic Acid And Vitamin C On Oxidative Stree And Inflammatory Cytokines In Patients With Type2 Diabetes

Objective 1. Observe total SOD activity, MDA content and the level of ICAM-1, hsCRP level changes in patients with type 2 diabetes after the short-term intensive insulin antidiabetic therapy. 2.Investigate the influence ofα-lipoic acid and vitamin C on oxidative stress and endothelial cell function in patients with type 2 diabetes after the intensive insulin antidiabetic therapy.Methods Serum total SOD activity, MDA content and the level of ICAM-1, hsCRP in patients of type 2 diabetic were tested before and after the treatment of 7 days intensive insulin antidiabetic treatment,and the patients were divided into three groups:⑴α-lipoic acid group, continuing intensive insulin antidiabetic treatment and addingα-lipoic acid treatment for 7 days before the targets were tested;⑵vitamin C group, continuing intensive insulin therapy and adding vitamin C treatment for 7 days before the targets were tested;⑶the control group, continuing intensive insulin antidiabetic treatment for 7 days before the targets were tested. Spectrophotometry was used to detect and calculate the total SOD activity, MDA content; ELISA was used to detect levels of ICAM-1, hsCRP levels.Results 1. Serum total SOD activity, MDA content and the level of ICAM-1, hsCRP levels were not significantly different inα-lipoic acid, vitamin C group before receiving intensive insulin antidiabetic treatment. 2. Serum SOD activity was significantly increased (P <0.01) and MDA significantly decreased (P <0.01) in 33 cases of patients receiving 7days intensive insulin therapy, and ICAM-1 levels, hsCRP levels were not significantly changed. 3. Comparison ofα-lipoic acid 0d,α-lipoic acid 7d serum total SOD activity, MDA content and the level of ICAM-1, hsCRP levels, total serum SOD activity was significantly increased (P <0.05), MDA significantly decreased (P <0.01) , ICAM-1 levels, hsCRP levels were not significantly changed inα-lipoic acid group which was added to theα-lipoic acid for 7days after 7days intensive insulin therapy. 4. Comparison of vitamin C 0d, vitamin C 7d serum total SOD activity, MDA content and the level of ICAM-1, hsCRP levels, total serum SOD activity was significantly increased (P <0.05), MDA significantly decreased (P <0.05), ICAM-1 levels hsCRP levels were not significantly changed in vitamin C group which was added to the vitamin C for 7days after 7days intensive insulin therapy. 5. Serum total SOD activity, MDA content and the level of ICAM-1, hsCRP levels have no significant difference in two groups treated withα-lipoic acid or vitamin C 7d.Conclusion 1, Correction of high blood sugar state with intensive insulin therapy can elevate serum total SOD activity and decrease MDA content in type 2 diabetic patients; 2, Compared with the total SOD activity and MDA content, no significant difference in intensive insulin therapy plusα-lipoic acid 7d or vitamin C 7d , antioxidant effect ofα-lipoic acid in short-term equal to vitamin C; 3, Intensive insulin therapy can reduce oxidative stress, but can not immediately reduce the level of ICAM-1 and hsCRP, improve endothelial function in the body; 4, Short-termα-lipoic acid or vitamin C treatment has a certain antioxidant capacity, but can not simultaneously reduce the body's levels of ICAM-1 and hsCRP, which does not improve endothelial function.