The Effect Of Musk-Aconite-Earthworm-pill On Chondrocyte Of IGF-Ⅰ, Type Ⅱ Collagen In Rabbits With KOA

Objective: Determined by immunohistochemical staining of rabbit knee osteoarthritis (KOA) knee cartilage IGF-Ⅰ(insulin-like growth factor) and typeⅡcollagen expression in chondrocytes , illustrating. Musk-Aconite-Earthworm-pill have a certain restorative and protective effect on articular cartilage, and to further clarify the drug in improving joint function and protection of articular cartilage in areas of possible mechanism, in order to provide experimental basis for clinical treatment.Methods:1.Divide group and build model: 40 healthy male rabbits (weight 2.5Kg±150g) were randomly divided into four groups: A. normal control group, B. model group, C. Musk-Aconite-Earthworm pill group, D. Anti-bone hyperostosis tablet group .Each group of 10. A group of rabbits convention raising .B, C, D group of each rabbit in the experiment 1st, 4th, 7th days injects 1.6% papain physiological saline solution 0.5ml establishment rabbit knee osteoarthritis model in the right hind legs knee joint cavity.2.Give medicine: Tests the 8th day to start for the medicine, C, D two groups of daily each one time, According to the adult each kilogram body weight every day medication quantity's 6 time of achievement rabbit each kilogram every day body weight's equivalent dosage, group C of each rabbit gives in the musk-aconite-earthworm pill 0.6g/kg /d, group D of each rabbit gives in anti-bone hyperostosis tablet 0.36g/kg /d. Dissolves fills the stomach in the 10ml physiological saline after the stomach tube. Groups A,B pour into the isometric physiological saline. Each group fills in the stomach for 4 weeks. Period convention raising , normal potable water. 3 After filling in the stomach finished namely-tests the 38th day, open the right knee joint to observe generally, cut and take the shinbone platform.4 detected the expression levels of IGF-Ⅰand typeⅡcollagen in cartilage tissue by Cartilage tissue section immunohistochemistry.Results:1. Observe the changes of pathomorphology by light microscope: Cartilage: Group A cartilage cell arranged orderly, there was no crazing and coloboma , no cell degeneration , hyperplasia, tidal line was integrity. Group B quantity of cartilage cell decreased obviously ,arranged disorderly, there was obvious ulcer in cartilage, tidal line were not integrity or broken. Group C and D coloboma were lesser than group B, quantity of cartilage cell increased than group B, arranged relatively orderly, tidal line was integrity.2 IGF-Ⅰ,Ⅱcollagen positive expression rate: B Group cartilage IGF-Ⅰ,Ⅱcollagen positive expression rate significantly lower than the A group (P <0.05), C group and D group of IGF-Ⅰ,Ⅱcollagen positive expression rate than the B group were significantly higher (P <0.05), C positive expression group was higher than D group (P> 0.05).Conclusion: Musk-Aconite-Earthworm-pill can increase the effective of the reparative growth factor type -ⅠandⅡin Experimental osteoarthritis of rabbit knee cartilage cell, and further clarify the drug in curing the osteoarthritis of the knee mechanism and the regulation of cytokine levels in cartilage have some relationship, moreover,provide directive function in the use of clinical medicine.