Study Of Role Of DAI As A Cytosolic DNA Sensor In Chronic Rhinosinusitis And Nasal Polyps Pathogen Mechanisms

PartⅠCytosolic DNA Sensor Expression in Chronic RhinoSinusitis and Nasal PolypsObjectiveTo study the expression of DAI (DNA-dependent activator of IFN-regulatory factors) mRNA in chronic rhinosinusitis (CRS) and nasal polyps (NPs) ,compared the DAI expression between chronic rhinosinusitis (CRS) and nasal polyps (NPs) patients, and to explored the role of DAI inthepathogenesis of chronic rhinosinusitis (CRS) and nasal polyps (NPs).MethodsThe mRNA expression of DAI in human bronchial epithelial cell line BEAS-2B and tissue were detected by RT-PCR. tissue DAI levels were compared among controls,CRS and NPs patients by means of real time RT-PCR and immunohistochemistry.ResultsThe DAI mRNA were expressed in BEAS-2B cells and tissue. The mRNA and protein expression rates of DAI were significant inhibited in both CRS and NPs patients.There were a significant further decrease of DAI expression in CRS patients.ConclusionDAI mRNA and protein were expressed in both patients and controls.Significant difference in DAI expression was found between patients and controls.Which may be one of the evidence that DAI may take part in the pathogenesis of CRS and NPs. PartⅡThe Effect of Double- Standed DNA on BEAS-2B Cells and Nasal Mucosal Tissue Explant CultureObjective1 To ensure wherther dsDNA can induced the expression of IFN-βand DAI.2 To explore the interaction between cytokines when BEAS-2B cells stimulated with dsDNA.3 Use of nasal mucosal Tissue explant culture study the effect of B-DNA on nasal mucosal tissue related cytokines, explore the role of DAI plays in acute exacerbation of CRS and NPs.Methods1 BEAS-2B cells were transfected with poly(dA-dT).poly(dT-dA). Cellular total RNAs were extracted and detected for related cytokines expression by reverse transcription real-time PCR. The expression level of DAI protein was detected by the techniques of western blot.2 Nasal mucosal Tissue explant culture were transfected with B-DNA, and subjected to reverse transcription real-time PCR analysis to evaluate the expression of related cytokines mRNA.Results1 dsDNA was able to induce type I interferon in BEAS-2B cells in a time- and dose-dependent manner. in the early stages, dsDNA-induced high expression of pro-inflammatory factor, and anti-inflammatory factor reached a much higher level in the late stage in BEAS-2B cells.2 After nasal mucosal Tissue explant culture were transfected with dsDNA, IL-6, IL-8, IP-10, IL-1βand MCP-3 mRNA was greatly high expressed in NPs, TNF-α, IL-17, IL-22 and IFN-λ2 was mainly high expressed in CRS, but was not the normal inferior turbinate mucosa, it have a lowest reactive . Conclusionafter BEAS-2B stimulated with dsDNA, the body via a negative feedback effect participates in the regulation of inflammatory responses, the anti-inflammatory is the ultimate representation of the feedback regulation. it provides protective effect on the body. when stimulated with pathogenic microorganisms, CRS and NPs may be more vulnerable than normal turbinate mucosa, leading to acute exacerbation of CRS and NPs. The effects mentioned above, it may work through dsDNA receptors DAI.