Analysis Of Cell Cycle's Correlation Of Phosphorylation Of H2AX

Objective: Analyzing and discussing the cell cycle's correlation of phosphorylation of H2AX(γ-H2AX), we can collect the datas for the further studies ofγ-H2AX. Methods: MOLT-4 cells, and peripheral blood lymphocytes(PBLs) with or without 48h culture of phytohemagglutinin(PHA), were irradiated by UV rays. Cell cycle and cell apoptosis were detected with sub-G1 peak method, fluorescence-labeled antibody ofγ-H2AX was used to detectγ-H2AX at the DNA double-strand breaks(DSBs) of chromatin, the damage of DNA was analyzed with flow cytometry, the expression ofγ-H2AX was detected with western blot. Results: Sub-G1 peak emerged apparently on the DNA histograms, and the cells of apoptosis increased gradually with the progression of time. The increase of the expression ofγ-H2AX emerged, and firstly raised, then decreased. PBLs with culture of PHA's 48h entered apparently cell cycle and the cells of S and G2/M phase emerged. PBLs without PHA's culture did not enter cell cycle. The increase of the expression ofγ-H2AX was showed with western blot, and the increase also firstly raised, then decreased. Conclusion: Phosphorylation of H2AX(γ-H2AX) expressed in the cells of stationary phase and proliferative phase. With the progression of time, the increase of the expression ofγ-H2AX firstly raised, and then decreased.