Approach Of Plasma Protein Markers In Deep Venous Thrombosis

Objective: To detect the plasma proteomic patterns using SELDI-TOF-MS ProteinChip array technique in lower-limb deep venous thrombosis,lower extremity varicose veins and normal control, screen biomarker candidates,build diagnostic models and evaluate its clinical significance in early diagnosis of thrombotic diseases.Methods: SELDI-TOF-MS ProteinChip was used to detect the plasma proteomic patterns of 46 cases with lower-limb deep venous thrombosis, 48cases with lower extremity varicose veins and 42 controls.The diagnostic models were developed by bioinformatics tool (nonlinear support vector machine, SVM), and the method of leave-one-out cross validation was used to estimate the discriminating power of patterns.Results : The modelâ… composed by 6 protein peaks could do the best in the diagnosis between lower-limb deep venous thrombosis and lower extremity varicose veins.Its ?sensitivity and specificity were 100%and 97.9%,respectively. The modelâ…¡composed by 3 protein peaks could do the best in the diagnosis between lower-limb deep venous thrombosis and normal control.Its sensitivity and specificity were 95.7%and 95%, respectively.Conclusion: SELDI-TOF-MS is a considerable sensitive and specific protein analytical method which may offer new approach to early diagnosis and differential diagnosis of deep venous thrombosis...