PELP1/MNAR And Ki67 Expression In Endometrial Cancer And Their Clinicalpathological Significance

Endometrial cancer (cancer of the uterine corpus) is one of the common malignant tumor of the female genital tract, accounted for 20% to 30% of the female genital tract malignant tumors.In developed countries, incidence rates for EMC ranks first in all malignant tumor of the female genital tract, mortality ranks second. Clinically, prognosis of early stage(stageâ… -â…¡) patients with EMC is good, the 5-year survival rate is close to 90%, and terminally stage patients(stageâ…¢-â…£) is only 15%. Therefore, accurately identifying high-risk EMC patients, judging correctly biological actions and prognosis, and looking for new individualized therapeutic targets are particularly important, which can greatly improve the EMC cure rate and reduce mortality. PELP1 / MNAR is a novel ER coactivator, can regulate the biological actions of ERs resulting from genomic and non-genomic signaling. It can promote cell proliferation through activating the Src/MAPK pathways and inhibits cell apoptosis via activating PI3K/Akt pathways. The study found that PELP1/MNAR overexpressed in EMC, is expected to become new tumor markers of EMC.Ki67 is a nuclei related antigen, which is widely used for detecting cell proliferation as a marker currently. This study was designed to evaluate the expression of PELP1/MNAR and Ki67 proteins in EMC,analys the relationship between PELP1/MNAR and Ki67, explore the relation of PELP1/MNAR and Ki67 expression with clinical pathology factor,including disease occurrence,development and metastasis etc, which provide the basis for deciding prognostic and individualized treatments.Methods1. A total of 76 paraffin-embedded endometrial cancer tissue blocks were obtained from the Department of Gynecologic Oncology at the Cancer Affiliated Hospital of Shantou University Medical College in the period between July 1999 and April 2009, and clinical data of these patients was collected.2. The immunohistochemical detection system was used to detect the expression of PELP1/MNAR protein and Ki67 in endometrial cancer.3. SPSS Statistics 17.0 software was used to analyze the relationship between the expression of PELP1/MNAR,Ki67 in EMC with clinical stage, histological grade, myometrial invasion and lymph node metastasis, as well as the relationship between PELP1/MNAR and Ki67.Results1. The positive rate of PELP1/MNAR protein and Ki67 in EMC were 100% and 68.4%.The patterns of PELP1/MNAR and Ki67 expression in the tumor cells were nuclear staining.2. The expression of PELP1/MNAR protein is positively correlated with the expression of Ki67 in EMC, P = 0.036,r=0.322.3. The relationship between PELP1/MNAR protein expression intensity and the clinicopathological significance of EMC:The intensity of PELP1 protein expression in advanced endometrial cancer (â…¢-â…£stage), was significantly higher than that in early ovarian cancer (â… -â…¡stage), P = 0.000.In moderately and poorly differentiated endometrial cancer (G2,G3),the intensity of PELP1 protein expression, was significantly higher than that in well differentiated endometrial cancer (G1), P = 0.027,P = 0.008. PELP1 protein expression intensity of endometrial cancer with deep myometrial invasion, was significantly higher than that in the superficial myometrial invasion ones, P = 0.000.In retroperitoneal lymph node positive endometrial cancer,the intensity of PELP1 expression was higher than the retroperitoneal lymph node negative ones,P=0.015.The intensity of PELP1 protein expression in endometrial cancer,which showed positive cancer cells in ascites or peritoneal washing liquid,was obviously higher than negative ones.P=0.000. The intensity of PELP1 protein expression in adnexa metastasis endometrial cancer was higher than opposite ones.P=0.000. However, the intensity of PELP1 protein expression in EMC was not related with the expression of ER, PR and menopause cases (P> 0.05).4. The relationship between Ki67 protein expression intensity and the clinicopathological significance of EMC: The level of Ki67 expression was related with tumor grade, clinical stage and clinical stage (P <0.05). However, the Ki67 expression levels in EMC was not related with myometrial invasion and retroperitoneal lymph node metastasis (P> 0.05).Conclusions1. PELP1/MNAR is expected as a marker of endometrial tumor progression.2. The intensity of PELP1/MNAR is positively correlated with the level of Ki67 in EMC, which suggest they may have a synergistic effect in the occurrence and development of endometrial cancer.3. The intensity of PELP1/MNAR and the levels of Ki67 expression were positively correlated with clinical stage of EMC. Suggest PELP1/MNAR and Ki67 may affect the progression of EMC.4. PELP1/MNAR and Ki67 were significantly related to malignant degree and metastasis in EMC,which was important to determining tumor biological behavior and prognosis. Detecting the two indicators can be used for screening high-risk group and offering reference for individualized therapy.