Expression Of Ki67,Caspase-3 And E-cad In Endometrial Carcinoma And Its Clinical Significance

Objective To study the expression of Ki67, cysteine aspartate-specific protease-3 (Caspase-3) and E-cadherin(E-cad) in endometrial carcinoma and investigate the relation between Ki67, Caspase-3 and E-cad.Methods Expression of Ki67 , Caspase-3 and E-cad protein were detetected by immunohistochemistry method (EnVision^TM method) respectively in 15 cases of atypical hyperplasia of endometrium and 68 cases of endometrial adenocarcinoma and compared with clinical characteristics, such as age, clinical-stage and pathological grade.20 case of normal endometrium tissues were investigated as control group.Results 1. The expressing situation of Ki67, Caspase-3 and E-cad : The expression of Ki67 protein in endometrial adenocarcinoma was significantly higher than in normal endometrium tissues (P<0.01). The expression of Caspase-3 and E-cad protein in endometrial adenocarcinoma was significantly lower than in normal endometrium tissues (P<0.01).2. The relation between Ki67 expression and the clinical pathological characters of endometrial adenocarcinoma:The positive rate of Ki67 was strongly associated with the clinical stage (P=0.007) and pathological grade (P<0.01) . There was no relationship between Ki67 protein expression and other clinical pathological characters (P>0.05).3. There was no relationship between Caspase-3 protein expression and the clinical pathological characters in this experiment.(P>0.05).4. The relation between E-cad expression and the clinical pathological characters of endometrial adenocarcinoma : The positive rate of E-cad was strongly associated with the clinical stage. (P=0.018) , histological grade (P=0.006) ,muscle invasion (P=0.025) and lymph node matastasis (P=0.019) .5. The relation between Ki67,Caspase-3 and E-cad: The expression of Ki67 was negatively associated with Caspase-3 (r=-0.407 , P=0.001) .The expression of Ki67 was negatively associated with E-cad (r=-0.365 , P=0.002) .But the expression of Caspase-3 was positivelyly associated with E-cad (r=0.244 , P=0.044) .Conclusions 1.The expressing level of Ki67 in normal endometrium was lower than atypicalhyplasia and endometrial adenocarcinoma tissues . The expressing level of Caspase^3 in normal endometrium and atypical hyplasia was higher than in endometrial adenocarcinoma . The expression level of E-cad in normal endometrium was higher than in atypical hyplasia and endometrial adenocarcinoma tissues .All the results were highly significant. The results suggest that the expressing changes of Ki67, Caspase-3 and E-cad may activate the oncogene and play an important role in the happen of endometrial carcinoma.2.High expression of Ki67 and low expression of E-cad protein were related to clinical stage and pathological grade in endometrial carcinomas. The results show that high expression of Ki67 and low expression of E-cad may relate with the development of endometrial adenocarcinoma predict poor prognosis.3.The expression of Ki67 was negatively associated with Caspase-3 and E-cad, but there was a positive correlation beween Caspase-3 and E-cad. They all play important roles in the carcinogenesis of endometrial carcinoma.Postgraduate student: Gao Zhanjun(Gynecology) Directed by Prof. Ji Xinqiang...