Application Research Of Capillary Electrophoresis In The Analysis Of Saikosaponin In Radix Bupleuri And The Compound Preparation

This thesis includes the following five chapters:Chaper 1This article is mainly introducing the physicochemical properties and pharmacological effects of Radix Bupleuris's main components.In this part,we also highlights the different analysis methods of Saikosaponin in Radix Bupleuri.It is narrated that the purpose and the main contents of this study.Chapter 2To establish a method for the determination of Saikosaponin a and Saikosaponin d of Radix Bupleuri by capillary electrophoresis. The separation was performed on an uncoated fused silica capillary of 60cm×50μm ID (53cm effective length). 20 mmol/L Tris +20 mmol/L SBE-β-CD+ 25 mmol/Lβ-CD buffer solution(pH9.93) was selected for the running buffer. The voltage applied was 15kV. The sample was injected by gravity(10s,15cm). The detection wavelength was 210 nm. The internal standard was p-nitrobenzoic acid. The linear ranges of determination for Saikosaponin a and Saikosaponin d were 60～300μg/ml(r=0.9997),20～100μg/ml(r=0.9996). The average recovery for Saikosaponin a was 102.3%, precision of the method was 1.89% (RSD,n=6). The average recovery for Saikosaponin d was 102.2%, precision of the method was 2.28% (RSD,n=6). The method proved to be convenient,rapid and well repeatable,and can be used for quantitative determination of the Radix Bupleuri .Chapter 3To establish a method for the determination of Saikosaponin a,d in Xiaochaihu Keli by capillary electrophoresis.The separation was performed on an uncoated fused silica capillary of 65cm×75μm ID (57cm effective length). 20 mmol/L Tris +20 mmol/L SBE-β-CD+ 25 mmol/Lβ-CD buffer solution(pH9.93)was selected for the running buffer. The voltage applied was 12kV. The sample was injected by gravity(10s,15cm). The detection wavelength was 210 nm. The internal standard was benzoic acid. The linear ranges of determination for Saikosaponin a and Saikosaponin d were 10.10～101.0μg/ml(r=0.9987),8～80μg/ml(r=0.9990). The average recovery for Saikosaponin a was 97.1%, precision of the method was 1.57% (RSD,n=6). The average recovery for Saikosaponin d was 98.1%, precision of the method was 1.98% (RSD,n=6). The method proved to be convenient,rapid and well repeatable,and can be used for quantitative determination of the preparation.Chapter 4To establish a method for the determination of Saikosaponin a,d in Xiaoyao Wan by capillary electrophoresis.The separation was performed on an uncoated fused silica capillary of 65cm×50μm ID (57cm effective length). 20 mmol/L Tris +20mmol/L SBE-β-CD+ 25 mmol/Lβ-CD buffer solution(pH9.93) was selected for the running buffer. The voltage applied was 10kV. The sample was injected by gravity(10s,15cm). The detection wavelength was 210 nm. The internal standard was p-nitrobenzoic acid. The linear ranges of determination for Saikosaponin a and Saikosaponin d were 20.16～201.6μg/ml(r=0.9971),10.2～102.0μg/ml(r=0.9947). The average recovery for Saikosaponin a was 99.4%, precision of the method was 2.46% (RSD,n=6). The average recovery for Saikosaponin d was 98.7%, precision of the method was 1.07% (RSD,n=6). The method proved to be convenient,rapid and well repeatable,and can be used for quantitative determination of the preparation.Chapter 5To establish a method for the determination of Saikosaponin a,d in Qizhiweitong Keli by capillary electrophoresis. The separation was performed on an uncoated fused silica capillary of 65cm×50μm ID (57cm effective length). 20 mmol/L Tris +20mmol/L SBE-β-CD+ 25 mmol/Lβ-CD buffer solution(pH9.93) was selected for the running buffer. The voltage applied was 12kV. The sample was injected by gravity(10s,15cm). The detection wavelength was 210 nm. The internal standard was p-nitrobenzoic acid. The linear ranges of determination for Saikosaponin a and Saikosaponin d were 20.16～201.6μg/ml(r=0.9953),16.26～162.6μg/ml(r=0.9986). The average recovery for Saikosaponin a was 97.9%, precision of the method was 1.28% (RSD,n=6). The average recovery for Saikosaponin d was 96.3%, precision of the method was 2.06% (RSD,n=6). The method proved to be convenient,rapid and well repeatable,and can be used for quantitative determination of the preparation.