| Bupleuri radix is a kind of apiaceae plant,which mainly contains saponins,volatile oil,polysaccharides and other active ingredients,and has antipyretic,liver protection,anti-inflammatory,anti-tumor,immune regulation,anti depression and other pharmacological activities.Bupleuri radix has been widely used for its great medicinal value and good clinical efficacy.there are about 549 varieties of chinese patent medicines containing Bupleuri Radix in china's current prescriptions,and 105 proprietary chinese medicines containing Bupleuri Radix are included in the 2015 edition and the supplementary edition of chinese pharmacopoeia.In terms of compound medicines,such as xiaochaihu decoction,which is one of the most widely used and most influential chinese preparations in japan;the traditional formula Soshiho-Tang having an important position in south korea.With the decrease of wild resources,at present,Bupleuri Radix is dominated by cultivated varieties.The varieties of Bupleuri Radix in the medicinal material market are confused and the quality is uneven,which affects its safety and quality stability.In addition,the quality of Bupleuri Radix decoction pieces directly affects the clinical efficacy of traditional chinese medicine,and it is more difficult to distinguish the characters of decoction pieces,so its quality and safety need to be strengthened.In this study,based on the traceability of the sample information source and the representativeness of the sample,TLC identification,moisture,ash,extract,saponin content of Bupleuri Radix decoction pieces were carried out;the volatile oil components of Bupleuri Radix were analyzed by GC-MS;the control mode of the characteristic map of Bupleuri Radix was established based on HPLC.In addition,a new method for the separation and quantitative determination of total saponins in bupleurum dropping pills by ultra-performance convergence chromatography(UPC~2)was established.The results show that there are some samples of Bupleuri Radix decoction pieces unqualified under different test items,which should be paid attention to.The test data can provide basic data for the quality standard of Bupleuri Radix.To some extent,the established control mode of characteristic spectrum of Bupleuri Radix can identify Bupleurum Chinense DC.and its common confusions.1.Some non-Bupleurum Chinense DC.species can be distinguished by the difference of the peak of the characteristic spectrum.2.by controlling the relative retention time of the characteristic peak,that is,the relative retention time of the index component peak saikosaponin d can be specified as 1,saikosaponin a relative retention time range was 0.51±0.05;saikosaponin c relative retention time range was 0.72±0.05,characteristic peak four relative retention time range was 1.57±0.1,excluding some of non-Bupleurum Chinense DC.varieties;3.sample identification was conducted by controlling the relative peak area ratio range to assist the characteristic map of Bupleuri Radix,that is,the ratio range of saikosaponin a(ssa)and saikosaponin d(ssd)could be defined as 0.52-1.25,and the ratio range of saikosaponin c(ssc)and saikosaponin a(ssa)was 0.13-0.35(the actual value fluctuated by20%).This control mode can provide reference for the quality research of Bupleuri Radix and other multi base traditional chinese medicine.In the separation and determination of saikosaponins,UPC~2 and HPLC were compared.The results showed that UPC~2 had obvious advantages in the separation of saikosaponin.In addition,the chromatographic conditions(mobile phase,column temperature,ABPR and formic acid concentration in mobile phase)of UPC~2 were systematically investigated and optimized,and the quantitative methodology was also investigated.The results showed that the separation efficiency of the method was high,five saikosaponins were separated successfully in 22 minutes,and the repeatability and recovery rates were satisfactory.This method can provide a new method for the separation and determination of saikosaponin in traditional chinese medicine. |
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