Association Study Of Polymorphisms Of P21^WAF1/CIP1 To Esophageal Cancer In Ningxia Han Population

Objective Esophageal Cancer (EC) is one of the most common archenteric malignant tumor in China. The mortality rate ranks fourth in malignant tumor. China has the high incidence of esophageal cancer including Ningxia autonomous region. The high incidence of Ningxia esophageal cancer is in accordance with the typical disease model of the interaction between multi-gene genetic susceptibility and environment. Codon 31 is one of the frequently reported codons in the coding region of p21WAF1/CIP1 gene. This subject that chosen codon 31 to study is order to find the relationship between codon 31 polymorphism,smoking, alcohol drinking and susceptibility of Ningxia esophageal cancer. This subject may provide some theoretical foundation of the genetic mechanism of Ningxia esophageal cancer, and provide reference of prevent , diagnosis and therapy as soon as possible in susceptible population of esophageal cancer in Ningxia.methods (1) Empirical approach: Collect 80 blood samples of Ningxia Medical University Affiliated Hospital of surgical resection specimens, confirmed by histopathology, were not carried out pre-operative radiotherapy and chemotherapy for case of esophageal cancer and 200 blood samples of health cases for control; frozen storage; extract DNA by Resin-based DNA extracted-kit; acquire specific fragment by PCR; direct sequencing of PCR products.(2) statistics: Analyze single genotype of SNP and allele frequency in case group and control group byχ2 test; analyze p21WAF1/CIP1 codon31 Polymorphisms and environmental factors in the risk of esophageal cancer by unconditional logistic regression model, and calculated OR value and 95% confidence interval.Result (1) Age and gender have no significant difference between case group and case group(P>0.05). Smokers and drinkers in case group were 44 (55.00%) and 23(28.75%), and in control group were 53(26.5%) and 29 (14.50%). Two groups had significant difference(P<0.05). (2) Smoking and drinking could increase the risk of esophageal cancer(OR = 3.222,95% CI = 1.860-5.580,P <0.05;OR = 2.215, 95% CI = 1.139–5.829,P<0.05). (3) The expression of Ser / Ser homozygote genotype had significant differences between the control group and the esophageal cancer group(35.00 vs. 21.00%,P<0.05), and this genotype could increase the risk of esophageal cancer(OR = 2.170, 95%CI = 1.180- 3.992, P <0.05).Conclusion (1) Codon31 genotype homozygous AGC is one of the risk factors of esophageal cancer in Ningxia Han population. (2) Smoking and Alcohol drinking may be the risk factors of esophageal cancer in Ningxia Han population.