| Objective: To observe the intervention during pregnancy, breast cancer, genistein (MMTV)-erbB-2 transgenic mice to their offspring mouse mammary tumor occurrence and development of impact and by immunohistochemical staining to analyze the potential mechanism of action.Methods: By genistein 20μg, 100μg dissolved in 2% DMSO (dimethyl sulfoxide), and 0.05ml vegetable oil in the control group was given equal amount of vegetable oil processing, the pregnant rats were randomly divided into low and high dose of genistein administration group and normal control group, n = 30, since the pregnancy the first 13 to 19 days using female mouse hind leg lateral subcutaneous injection, female mouse in all experimental groups observed and recorded the number of deliveries, the number of each winter pen mouse, and the female ratio, as well as the after the birth of their offspring in mice the first day 2,15,30 weight change was observed in the intervention group genistein offspring of female offspring of female mice and the control group the incidence of mammary tumors in mice, the incubation period and tumor diameter and by HE staining and immunohistochemical staining was detected by SP progeny mouse mammary tumor histological type, and the expression of P53 to make statistical analysis.Results: Genistein during pregnancy intervention group compared with the blank control group, the number of each winter pen mouse, and the female ratio and weight gain after the birth of the child rats no significant effect (P>0.05); high-dose group, low-dose group and the control group of small The tumorigenic mouse mammary tumor rates were 27%, 60% and 77%, high-dose group and other groups decreased significantly compared with the incidence (P<0.05), while the low-dose group and control group difference was not statistically significant (P>0.05); high-dose group, low-dose group and control group, the incubation period of mammary tumors in mice, respectively (47.1±5.2) weeks, (38.3±4.7) weeks, and (37.7±4.6) weeks, high-dose group than in the control group latency was significantly longer (P<0.05), low-dose group than in the control group no significant difference in latency (P>0.05), high-dose group the lower dose group no significant difference in latency (P>0.05); high-dose group, low-dose group and control group mouse mammary tumor diameter compared with no significant difference (P>0.05); P53 in breast cancer tissues and normal breast tissues were positive expression rate of 67% and 26.5%, the difference was statistically significant (P<0.05), high-dose group, low-dose group and control group mouse mammary tumor tissue P53 positive expression rates were 37.5%, 72.2% and 73.9%, high-dose group than in the other groups was significantly lower expression (P<0.05 ), Low-dose group and the control group no statistically significant difference (P>0.05).Conclusion: MMTV-erbB-2 in transgenic mice in our breeding, growing well, with a strong breeding and adaptability; during pregnancy, the use of isoflavone on the winter pen mouse progeny mice per the number of female ratio as well as their offspring mice were born After body weight had no significant effect; application of high doses of isoflavones can reduce the incidence of breast cancer, to extend the offspring of female mice with tumors of the incubation period, and can reduce the offspring of mice with breast tumor levels of positive expression of P53, suggesting that genistein of breast cancer (MMTV)-erbB-2 transgenic mouse mammary tumors have a certain preventive effect.
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