Preliminary Study On Extraction Of Periplane Americana Induce Endothelical Cell Migration

Endothelial cell migration play improtant role in many physiological and pathologic process.The integrity of vessel endothelium and angiogenesis depend on endothelial cell migration:in the process of wound healing,neovascularization by budding or other way is the consquence of the proliferous EC migrate to the breakage of vessel;in the elementary step of tissue repair,the capillary network in original state supplying granulation tissue with nutrientsubstance is the consquence of the proliferous EC migrate to anagenetic granulation tissue;additionally,the migration of EC is prerequisite for tumorigeness. Research on what chinese medicine induce EC migrate will be make contribution to clinic. The extraction from periplaneta americana were ofen used for treatment of wound healling and gastric ulcer effectively.There are a great deal of research on mechanism of periplaneta americana for wound healling,such as it adjust the secretory volume of EC,incerase the quantity of neutrophilic granulocyte, advance the endocytosis-ability of macrophage or promote the SOD level rally. But rerely research on what will happen to extraction of periplaneta americana act on EC. Therefore, in our reserch get help from tradional laboratory facilities and micro-fluidic chip to prove the chemotaxis of endothelial cells in response to the extraction from periplaneta americana.The solution is extract from periplaneta americana then using the traditional methods of scratch assay , transwell chamber and agarose plate test the extraction of periplaneta americana in various consistency induce EC migrate. A microfluidic device which using withdrawing thread form agarose hydrogel was designed and fabricated to generate stable concentration gradients immediately . Using this device monitored the chemotactic response of endothelial cell to the extraction of periplaneta americana.The methods of scratch assay and transwell chamber approved conformably that extraction of periplaneta americana can induce EC migrate: the extraction of medium and periplaneta americana volume ratio at 5% were unable to induce human endothelial cells migration. In contrast, to the extent that volume ratio is 10% to 30%, the relation of extraction concentration and the quantity of EC migration is linear dependence. There are respectively 7% and 3% of human endothelial cells were observed to migrate towards higher concentrations when the Medium and Periplaneta americana volume ratio was 20%.