| Objective: Constructing pAAV-CD151 plasmid and pAAV-CD151-AAA194-196 mutant (QRD), the vectors for pAAV-CD151, pAAV-CD151-AAA194-196 and pAAV-GFP were prepared using a triple-plasmid co-transfection method in 293 cell lines to paking rAAV, and then transfected into HUVEC, then study the Mechanism of Integrinβ1 promotes the proliferation and migration of human umbilical vein endothelial cell through CD151.Methods: Small plasmid extraction, the vectors for pAAV-CD151, pAAV-CD151-AAA194-196 and pAAV-GFP were prepared using a triple-plasmid co-transfection method in 293 cell lines to paking rAAV, the titer of rAAV was determined by the quantitative DNA dot-blot hybridization. Then transfected into HUVEC, HUVEC were divided into four groups: normal control group, group of green fluorescence (GFP group). The proliferation activity of HUVEC was measured by SRB assay; the ability of cell migration was detected by scratch test; the expression of CD151, Akt, P-Akt, PI3K, andβ1 were tested by Western Blot.Results: The CD151 group could significantly promote cell proliferation and migration compared with the control group and GFP group (P <0.05), while the QRD group significantly inhibited cell proliferation and migration . Transfection of pAAV-CD151 or pAAV-CD151-AAA194-196 mutant increased expression of CD151 in HUVEC. The expression of P-Akt, PI3K, andβ1 of CD151 group was significantly increased compared with control group ,GFP group and QRD group (p<0.05) , while the expression of total AKT in the four group has no statistical significance (p>0.05).Conclusion: CD151 plays an important role in promoting angiogenesis. The molecular mechanism involving may be that CD151 up-regulates the expression of PI3K by integrinβ1, which promotes the phosphorylation of Akt and enhance the vitality of Akt. This effect promotes endothelial cell migration and proliferation, as well as tubular structures formation, thus promoting angiogenesis.
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