| The analysis of antibiotics and other drugs is of importance for clinical application and pharmacokinetic studies. In this paper, the simple mode of the capillary electrophoresis -capillary zone electrophoresis method is developed for the simutaneous determination of some cephalosporins, fluoroquinolones and hemostatic drugs in biological fluids. It includes four chapters as follows:Chapter one: The capillary electrophoresis technique was described simply. The analytical research and development status of cephalosporin represented by cefazolin sodium and cefminox sodium; fluoroquinolones represented by gatifloxacin and hemostatic drugs represented by aminomethylbenzoic acid and etamsylate were reviewed.Chapter two: A capillary zone electrophoresis method was developed for the effective separation and sensitive detection of cefazolin sodium and cefminox sodium in rubbit surum. The effects of species, pH and concentration of buffer, voltage and the detection wavelength were investigated. Under the optimized condition, both of the linearity were satisfactory in the range of 5.12–256μg·mL-1. The limit of detection (LOD) was 1.45 and 1.26μg·mL-1, respectively. The limit of quantification (LOQ) was 4.8 and 4.2μg·mL-1, respectively.Chapter three: A novel and simple method was, for the first time, developed for effective separation and sensitive detection of aminomethylbenzoic acid, cefminox sodium and etamsylate in human urine by capillary zone electrophoresis with diode array detection (CZE-DAD). Sample handling methods, buffer species, pH, concentrationand voltage as well as detection wavelength were examined to optimize the working conditions of electrophoresis. Under the optimized condition, the LODs were 1.04, 2.08 and 1.04μg·mL-1 for aminomethylbenzoic acid, cefminox sodium and etamsylate, respectively, and their LOQs were 3.47, 6.93 and 3.47μg·mL-1.Chapter three: A novel and simple method was, for the first time, developed for effective separation and sensitive detection of gatifloxacin, aminomethylbenzoic acid, cefazolin sodium and cefminox sodium in human urine by capillary zone electrophoresis with diode array detection (CZE-DAD). Sample handling methods, buffer species, pH, concentration and voltage as well as detection wavelength was examined.to optimize the working conditions of electrophoresis Under the optimized condition, the LODs were 5.88, 3.90, 7.80 and 15.0μg·mL-1 for gatifloxacin, aminomethylbenzoic acid, cefazolin sodium and cefminox sodium, respectively, and Their LOQs were 19.6, 13.0, 26.0 and 50.0μg·mL-1.
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